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Anti γ h2ax

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The Anti-γ-H2AX product is a laboratory reagent used to detect the presence of phosphorylated histone H2AX, a marker of DNA double-strand breaks. This antibody-based product enables researchers to study the DNA damage response in cellular systems.

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Lab products found in correlation

Anti rad51, by Santa Cruz Biotechnology (3 mentions) Anti γ tubulin, by Merck Group (2 mentions) Anti brca2, by Santa Cruz Biotechnology (2 mentions) Anti tubulin, by Abcam (1 mentions) Anti ku86, by Santa Cruz Biotechnology (1 mentions) Anti phospho p53 ser15, by Santa Cruz Biotechnology (1 mentions) Anti fanci, by Santa Cruz Biotechnology (1 mentions) Anti fancd2, by Santa Cruz Biotechnology (1 mentions) Anti fanci, by Fortis Life Sciences (1 mentions) Anti p21, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by GeneTex (1 mentions) Dapi fluoromount g, by Southern Biotech (1 mentions) Deltavision elite imaging system, by GE Healthcare (1 mentions) Anti rif1, by Fortis Life Sciences (1 mentions)

4 protocols using anti γ h2ax

1

IR-Induced DNA Damage Foci and Centrosome Analysis

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IR-induced foci formation assay was performed as described previously.1 (link) The primary antibodies used here were anti-GST-Mcph1 (gift from Dr. Shiaw-Yih Lin at MD Anderson Cancer Center, Houston, Texas), anti-γ-H2AX (Bethyl Laboratories, Montgomery, TX, USA; Cat# A300-081), anti-Rad51 (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat# sc-8349), and anti-Brca2 (Santa Cruz Biotech, Santa Cruz, CA, USA; Cat# sc-28235). Centrosome analysis was performed as described elsewhere,66 (link) with primary antibody anti-γ-Tubulin (Sigma, St. Louis, MO, USA; Cat# T6557). Centrosome numbers were counted in approximately 300 cells from at least 10 fields per type of cells. These experiments were repeated at least three times.
Liang Y., Gao H., Lin S.Y., Goss J.A., Du C, & Li K. (2014). Mcph1/Brit1 deficiency promotes genomic instability and tumor formation in a mouse model. Oncogene, 34(33), 4368-4378.
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2

Antibody Detection in DNA Damage Repair

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The following antibodies were used for western blotting and immunofluorescence assays: anti-FANCD2 (Santa Cruz, sc-20022 and Abcam, ab2187), anti-FANCI (Bethyl Laboratories, A300–212), anti-FANCI (38 (link)), anti-Ku86 (Santa-Cruz, sc-5280), anti-CtIP (39 (link)), anti-RAD51 (Santa Cruz sc-8349), anti-GAPDH (Genetex, GTX627408), anti-tubulin (Abcam, ab7291), anti-γ-H2AX (Bethyl Laboratories, A300–081), anti-phospho-p53 (Ser15) (Santa Cruz, sc-11764-R), anti-p21 (Santa Cruz, sc-397) and anti-PCNA (Calbiochem, PC10).
Thompson E.L., Yeo J.E., Lee E.A., Kan Y., Raghunandan M., Wiek C., Hanenberg H., Schärer O.D., Hendrickson E.A, & Sobeck A. (2017). FANCI and FANCD2 have common as well as independent functions during the cellular replication stress response. Nucleic Acids Research, 45(20), 11837-11857.
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3

Quantifying DNA Damage Response by Immunofluorescence

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Immunofluorescence experiments were performed according to the recommended instructions of the Fast ImmunoFluorescence Staining Kit (Protein Biotechnologies). Briefly, cells were transiently transfected with scrambled control shRNA (SCR) or RIF1 knockdown shRNA (RIF1 KD) followed by treated with cisplatin (10 μM) and immunostained with anti-RIF1 (red) and anti-γ-H2AX (green) antibodies. Nucleus was stained with 4'6-diamidino-2-phenylindole (DAPI). The primary antibodies were listed as follows: anti-RIF1 (Bethyl Laboratories and Santa Cruz) and anti-γ-H2AX (Bethyl Laboratories). The secondary antibodies used were Alexa Fluor 488 and 594 conjugated second antibodies (Jackson ImmunoResearch). DAPI Fluoromount-G® (SouthernBiotech) was applied to detect the nucleus. The pictures were taken using DeltaVision Elite Imaging System (GE Healthcare Life Sciences). The relative fluorescence intensity was analyzed with Image J.
Mei Y., Liu Y.B., Hu D.L, & Zhou H.H. (2018). Effect of RIF1 on response of non-small-cell lung cancer patients to platinum-based chemotherapy by regulating MYC signaling pathway. International Journal of Biological Sciences, 14(13), 1859-1872.
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4

IR-Induced DNA Damage Foci and Centrosome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
IR-induced foci formation assay was performed as described previously.1 (link) The primary antibodies used here were anti-GST-Mcph1 (gift from Dr. Shiaw-Yih Lin at MD Anderson Cancer Center, Houston, Texas), anti-γ-H2AX (Bethyl Laboratories, Montgomery, TX, USA; Cat# A300-081), anti-Rad51 (Santa Cruz Biotechnology, Santa Cruz, CA, USA; Cat# sc-8349), and anti-Brca2 (Santa Cruz Biotech, Santa Cruz, CA, USA; Cat# sc-28235). Centrosome analysis was performed as described elsewhere,66 (link) with primary antibody anti-γ-Tubulin (Sigma, St. Louis, MO, USA; Cat# T6557). Centrosome numbers were counted in approximately 300 cells from at least 10 fields per type of cells. These experiments were repeated at least three times.
Liang Y., Gao H., Lin S.Y., Goss J.A., Du C, & Li K. (2014). Mcph1/Brit1 deficiency promotes genomic instability and tumor formation in a mouse model. Oncogene, 34(33), 4368-4378.
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