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2 protocols using sulfa trimethoprim

1

Antibiotic Susceptibility of Streptococcus and Staphylococcus

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Clinically isolates of Streptococcus suis (3 isolates) and Staphylococcus intermedius (3 isolates) were obtained from Microbiological Laboratory, Veterinary Diagnostic Center, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, Thailand. The bacteria strains were isolated and characterized by the differential bacterial culture and biochemical assays for clinical samples according to the standard method of Baron et al. (1994) [11 ]. S. suis were maintained in Microbank Cryovials and kept at −80°C. Staphylococcus intermedius were maintained in skimmed milk at −40°C until being used. Blood agar was obtained for Bacteriology Unit. Antibiotic discs, Amoxicillin/clavulanic acid 30 μg, Doxycycline 30 μg, and Sulfa-trimethoprim 25 μg were purchased from Oxoid, UK. Fosfomycin 50 μg was purchased from Bedson Co., Argentina. Chettaphanin I 10 mg/mL was used as control standard.
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2

Antimicrobial Susceptibility Testing

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An AST was performed using the Kirby-Bauer disc diffusion method according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) [4 ] with a 24-h incubation period at 35℃. Discs with the following compounds were used: amoxicillin-clavulanic acid, cefoxitin, ceftriaxone, cephazolin, chloramphenicol, clindamycin, penicillin, cloxacillin, doxycycline, gentamycin, oxytetracycline, sulfa-trimethoprim, tetracycline, and vancomycin (Oxoid).
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