Log In Sign up
The largest database of trusted experimental protocols

Annexin 5 pe

Manufactured by Miltenyi Biotec
Visit Supplier
Sourced in Germany

Annexin V-PE is a fluorescent-labeled protein that binds to phosphatidylserine, a phospholipid that is exposed on the surface of cells undergoing apoptosis. It is a tool used in flow cytometry and other cell-based assays to detect and quantify apoptotic cells.

Automatically generated - may contain errors

Lab products found in correlation

Macsquant analyzer 10 flow cytometer, by Miltenyi Biotec (2 mentions) Flowlogic, by Miltenyi Biotec (2 mentions) Propidium iodide solution, by Miltenyi Biotec (1 mentions) Macsquant analyzer 10, by Miltenyi Biotec (1 mentions) Rea pe, by Miltenyi Biotec (1 mentions) Cd11b fitc, by Miltenyi Biotec (1 mentions) Siglecf pe vio770, by Miltenyi Biotec (1 mentions) Gr 1 pe, by Miltenyi Biotec (1 mentions) Cd19 pe vio770, by Miltenyi Biotec (1 mentions) Novoexpress, by Agilent Technologies (1 mentions) Cytexpert software, by Beckman Coulter (1 mentions) Cd3 apc vio770, by Miltenyi Biotec (1 mentions) Cd45 pe, by Miltenyi Biotec (1 mentions) Cd45 vioblue, by Miltenyi Biotec (1 mentions) Viobility 405 520 fixable dye, by Miltenyi Biotec (1 mentions) Siglecf apc, by Miltenyi Biotec (1 mentions) Gr 1 apc, by Miltenyi Biotec (1 mentions) Cd11c pe, by Miltenyi Biotec (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Gallios 10 3 flow cytometer, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Annexin V (17 citations)

6 protocols using annexin 5 pe

1

Tumor Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
B16-F10 cells isolated from mouse tumor tissues were tested for viability. Viability of tumor cells in control and treatment conditions was evaluated using Annexin V-PE (Miltenyi Biotec®, Bergisch Gladbach, Germany) and PI staining. The stained cells were acquired using a MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec®), and the data were processed using Flowlogic (Miltenyi Biotec®).
Calvani M., Bruno G., Dabraio A., Subbiani A., Bianchini F., Fontani F., Casazza G., Vignoli M., De Logu F., Frenos S., Filippi L, & Favre C. (2020). β3-Adrenoreceptor Blockade Induces Stem Cells Differentiation in Melanoma Microenvironment. International Journal of Molecular Sciences, 21(4), 1420.
+ Open protocol
+ Expand
2

Multicolor Flow Cytometry of Tumor-Derived Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells isolated from mouse tumors were incubated and stained with various appropriately diluted (Supplementary Table S1) combinations of the following fluorochrome-conjugated antibodies: anti-CD45-VioBlue or VioGreen (130-110-802, 130-102-776), anti-CD11b-FITC or PerCP Vio700 (130-109-289), anti-Gr1-PE (130-102-426), anti-CD106-PE (130-104-712), anti-Sca1-PE Vio700 (130-106-220), anti-CD73-FITC (130-102-535), anti-CD29-APC (130-102-557), anti-CD44-VioBlue (130-116-495), anti-CD34-FITC (130-105-831), anti-CD31-PE (130-102-608), anti-CD140 (PDGFR alpha)-APC Vio 770 (130-105-119), anti-Ter119-APC (130-102-290), Lineage Cell Detection Cocktail-Biotin-APC Vio 770 (130-092-613), anti-CD49b-PE (130-102-337), anti-Ly6C-FITC (130-111-777), anti-Ly6G-APC (130-102-295), anti-CD117-PE (130-111-615), anti-CD135-APC (130-102-512), anti-CD127-APC (130-102-529), anti-CD122-FITC (130-102-481), Annexin V-PE (130-108-112), and Propidium Iodide Solution (130-093-233) (Miltenyi Biotec®, Bergisch Gladbach, Germany). The stained cells were acquired using a MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec®), and the data were processed using Flowlogic (Miltenyi Biotec®).
Calvani M., Bruno G., Dabraio A., Subbiani A., Bianchini F., Fontani F., Casazza G., Vignoli M., De Logu F., Frenos S., Filippi L, & Favre C. (2020). β3-Adrenoreceptor Blockade Induces Stem Cells Differentiation in Melanoma Microenvironment. International Journal of Molecular Sciences, 21(4), 1420.
+ Open protocol
+ Expand
3

Apoptosis Assessment by Annexin V-PE

Check if the same lab product or an alternative is used in the 5 most similar protocols
Apoptosis was assessed using Annexin V-PE (Miltenyi Biotec) as described in the Supplementary Information.
Yiangou L., Blanch-Asensio A., de Korte T., Miller D.C., van Meer B.J., Mol M.P., van den Brink L., Brandão K.O., Mummery C.L, & Davis R.P. (2022). Optogenetic Reporters Delivered as mRNA Facilitate Repeatable Action Potential and Calcium Handling Assessment in Human iPSC-Derived Cardiomyocytes. Stem Cells, 40(7), 655-668.
+ Open protocol
+ Expand
4

Red Blood Cell Membrane Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phosphatidylserine (PS) exposure at the outer membrane leaflet of RBCs and CD47 were assessed using Annexin V-PE (Miltenyi, 130-118-363) and anti-CD47-PE antibody (Miltenyi 130-101-348), respectively. Briefly blood samples were centrifuged at 1000×g for 5 min at RT. RBCs pellets were washed in PBS and resuspended at 0.2% Hct in the appropriate staining buffer according to manufacturer instructions. RBCs suspensions were incubated with Annexin-V-PE (1:11 dilution) or CD47-PE (1:34 dilution) for 20 min in the dark at RT. Unstained RBCs and isotype control (1:51 dilution, REA-PE, Miltenyi) were used as negative controls for PS exposure and CD47 levels, respectively. After incubation, samples were washed twice in their respective staining buffer and analyzed by flow-cytometry (MACSQuant Analyzer 10, Miltenyi). Gating was performed on 100,000 RBCs per condition.
Robert M., Laperrousaz B., Piedrahita D., Gautier E.F., Nemkov T., Dupuy F., Nader E., Salnot V., Mayeux P., D'Alessandro A., Lavazec C., Joly P., Scheer A., Connes P, & Cibiel A. (2021). Multiparametric characterization of red blood cell physiology after hypotonic dialysis based drug encapsulation process. Acta Pharmaceutica Sinica. B, 12(4), 2089-2102.
+ Open protocol
+ Expand
5

Flow Cytometry Analysis of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The differential immune cell count was determined by using either NovoCyte or Beckman Coulter CytoFLEX flow cytometers and analyzed with NovoExpress (Acea Biosciences, USA) or CytExpert software (Beckman Coulter, USA). Differential immune cell counts were performed as previously described (Chan et al. 2016 ). Leukocytes were identified as CD45+, alveolar macrophages as CD11c+Siglec-F+, eosinophils as CD11c−Siglec-F+, neutrophils as GR-1+CD11b+ or Ly-6G+CD11b+, B cells as CD3−/CD19+ and T cells as CD3+/CD19− cells. All antibodies were from Miltenyi Biotech: CD45-VioBlue (130–110-802), CD45-PE (130–110-797), CD64-APC-Vio770 (130–118-685), Siglec-F-PE-Vio770 (130–112-334), Siglec-F-APC (130–102-241), CD11b-PE-Vio615 (130–113-807), CD11b-FITC (130–098-085), CD11c-PE-PerCP700 (130–110-842), CD11c-PE (130–102-799), Ly-6G-PerCP700 (130–117-500), Gr-1-PE (130–112-306), Gr-1-APC (130–102-833), CD3-APC-Vio770 (130–119-793), CD19-PE-Vio770 (130–111-885), Annexin-V-PE (130–118-363), and Viobility 405/520 Fixable Dye (130–109-814).
Nguyen N., Xu S., Lam T.Y., Liao W., Wong W.S, & Ge R. (2022). ISM1 suppresses LPS-induced acute lung injury and post-injury lung fibrosis in mice. Molecular Medicine, 28, 72.
+ Open protocol
+ Expand
6

Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA content measurement for analyzing cell cycle parameters was performed according to Kaltschmidt and colleagues [34 (link)] by harvesting 1 x 106 cells at 300 g for 5 min followed by fixation with 70% (v/v) ethanol. After centrifugation at 300 x g for 10 minutes, staining solution (PBS containing 1 mg/ml glucose (Carl Roth GmbH), 4´,6-diamidino-2-phenylindole (DAPI; 0.5 mg/ml; Sigma-Aldrich), and 100 Kunitz units RNaseA (Thermo Fisher Scientific) was applied for 60 min under exclusion of light.
For apoptosis measurement, 1 x 106 cells were labeled with Annexin V-PE (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions. For analysis of H2B-mCherry, 1 x 106c-REL+/+ and c-REL-/- cells were harvested and directly applied for flow cytometric analysis without additional staining procedures.
DAPI or Annexin V-PE-labeled cells as well as unstained cells (H2B-mCherry) were analyzed using a Gallios™ 10/3 flow cytometer (Beckman Coulter, Brea, CA, USA). Data analysis was performed using FlowJo Software (TreeStar, Olten, Switzerland), doublet discrimination for cell cycle analysis was assured by appropriate gating strategies.
Slotta C., Schlüter T., Ruiz-Perera L.M., Kadhim H.M., Tertel T., Henkel E., Hübner W., Greiner J.F., Huser T., Kaltschmidt B, & Kaltschmidt C. (2017). CRISPR/Cas9-mediated knockout of c-REL in HeLa cells results in profound defects of the cell cycle. PLoS ONE, 12(8), e0182373.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!