Mouse melanoma cell line B16F0 (ATCC catalogue number CRL-6322™) was cultured in RPMI 1640 media, containing 10% (v/v) foetal bovine serum (FBS) (Bovogen Biologicals, Melbourne, Australia), 200 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin (Life Technologies, CA, USA). Cells were incubated in a T-75 flask at 37 °C, in a 5% CO2 incubator and were split every two days. The normal human dermal fibroblast (NHDF) cell line (PromoCell, Heidelberg, Germany) was cultured in fibroblast basal media 2 (PromoCell, Heidelberg, Germany), containing 2% (v/v) FBS, 1 ng/mL basic fibroblast growth factor and 5 μg/mL insulin (PromoCell, Heidelberg, Germany). Cells were incubated in a T-75 flask at 37 °C, in a 5% CO2 incubator, and split every two days.
Normal human dermal fibroblast cell line
Manufactured by PromoCell
Sourced in Germany
Normal human dermal fibroblast (NHDF) cell line is a primary cell type derived from healthy human skin. Fibroblasts are the most common cells found in connective tissue and play a crucial role in extracellular matrix production and wound healing.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Basic fibroblast growth factor (bfgf), by PromoCell (1 mentions)
Foetal bovine serum (fbs), by Bovogen (1 mentions)
Insulin, by PromoCell (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (1 mentions)
Pluronic f68, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Tryple, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions)
Penicillin streptomycin amphotericin b mixture, by Lonza (1 mentions)
2 protocols using normal human dermal fibroblast cell line
1
Culturing Mouse Melanoma and Human Fibroblast Cell Lines
2
Biocompatible Polymer Compound Analysis
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Hydroxypropyl cellulose (Klucel LF Mw 95,000 Da, 99% purity) was purchased from Aqualon, Hercules Inc. (Wilmington, USA), and used as received. Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (Pluronic F68) was purchased from Sigma-Aldrich USA as PEO76PPO29PEO76 (Mn ≅ 8400 g/mol, ≥99% HPLC, with 85% PEO groups and without additives (phenol red)) and used as received. The materials used for the biocompatibility study were: a normal human dermal fibroblast (NHDF) cell line purchased from PromoCell(Heidelberg, Germany); Eagle’s Minimal Essential Medium Alpha (aMEM) and 1% penicillin–streptomycin–amphotericin B mixture (10K/10K/25 µg in 100 mL), purchased from Lonza Verviers, Belgium); fetal bovine serum (FBS), purchased from Biochrom GmbH (Germany); Tryple, purchased from Gibco(Langley, VA, USA); phosphate buffered saline (PBS),purchased from Invitrogen (Eugene, OR, USA);and a CellTiter 96®AQueous One Solution Cell Proliferation Assay, purchased from Promega(Madison, WI, USA). All materials intended for cell culture tests were used as received, without additional purification, based on the specifications of use for cell studies.
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