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Talos f200s electron microscope

Manufactured by Thermo Fisher Scientific

The Talos F200S electron microscope is a high-resolution analytical instrument designed for materials science and life science applications. It features a field emission source and advanced optics to provide detailed imaging and analysis of samples at the nanoscale level.

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2 protocols using talos f200s electron microscope

1

Characterization of Nanoparticle Properties

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An FEI Talos F200S electron microscope was used to obtain TEM images, and the corresponding X-ray energy-dispersive spectroscopy (EDS) spectrum was acquired on the same electron microscope performed at 200 kV. The DLS and zeta potential were measured with a Zetasizer Nanoseries (Nano ZS90, Malvern Instrument Ltd) using a distilled water environment at 20°C. ASAP2460 (Micrometrics Instrument Corp) was used to determine the BET surface area and pore-size distribution with N2 adsorption–desorption technique analysis (Degassing time 6 h, degassing temperature 120°C). A UV-1900i Shimadzu spectroscope with QS-grade quartz cuvettes was used to collect UV-vis-NIR absorption spectra at ambient temperature.
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2

Multimodal Characterization of Nanomaterials

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Fluorescence spectra were recorded using a RF-6000 fluorescence spectrophotometer (Shimadzu, Japan). UV-vis spectra were collected using a UV-2450 spectrophotometer (Shimadzu, Japan). Dynamic light scattering (DLS) analysis was performed using a Nano-ZS Zetasizer ZEN3600 instrument (Malvern Instruments Ltd, UK). The infrared spectra were recorded using an IRAffinity-1 Fourier transform infrared spectrometer (Shimadzu, Japan). Transmission electron microscopy (TEM) images were measured using an FEI Talos F200S electron microscope (FEI, America). The morphology of the products was determined using an SEM (FESEM, S-8010, Hitachi). Fluorescence imaging was performed using a confocal laser scanning microscope (Nikon ALR, Japan). The vitro cytotoxicity assay was recorded on BioTek Epoch (BioTek Instruments, USA) at room temperature using 96-microwell plates.
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