Dionex ultimate 3000rs uhplc instrument

The chemical composition of the extracts was determined using a Dionex Ultimate 3000RS UHPLC instrument (Thermo Fischer Scientific Inc., New York, NY, USA). Samples were filtered (0.22 μm PTFE filter membrane, Labex Ltd., Budapest, Hungary) before HPLC analysis and injected onto a Thermo Accucore C18 (100 mm × 2.1, mm i. d., 2.6 μm) column thermostated at 25 °C (±1 °C). The solvents used were water (A) and methanol (B), acidified with 0.1% formic acid, and the flow rate was maintained at 0.2 mL/min. A gradient elution was used: 5% B (0–3 min), a linear gradient increasing from 5% B to 100% (3–43 min), 100% B (43–61 min), a linear gradient decreasing from 100% B to 5% (61–62 min) and 5% B (62–70 min). The column was coupled with a Thermo Q-Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) equipped with an electrospray ionization source. Spectra were recorded in positive and negative-ion mode, respectively. The trace finder 3.1 (Thermo Scientific, USA) software was applied for target screening. Most of the compounds were identified based on previously published studies or data found in the literature. The exact molecular mass, isotopic pattern, characteristic fragment ions, and retention time were always used to identify the molecules.

Compositions of the different extracts were determined using a Dionex Ultimate 3000RS UHPLC instrument(Thermo Scientific, Waltham, MA, USA). The extract was filtered through a 0.22 μm PTFE filter membrane (Labex Ltd., Budapest, Hungary) before HPLC analysis. Extracts were injected onto a Thermo Accucore C18 (100 mm × 2.1, mm i. d., 2.6 μm) column themostated at 25 °C (±1 °C). The solvents used were water (A) and methanol (B). Both were acidified with 0.1 % formic acid. The flow rate was maintained at 0.2 mL min⁻¹. The elution gradient was isocratic 5 % B (0–3 min), a linear gradient increasing from 5% B to 100% (3–43 min), 100% B (43–61 min), a linear gradient decreasing from 100% B to 5% (61–62 min) and 5 % B (62–70 min). The column was coupled with a Thermo Q-Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) equipped with electrospray ionization source. Spectra were recorded in positive- and negative-ion mode [21 (link)].

The chemical composition of the extracts was determined using a Dionex Ultimate 3000RS UHPLC instrument (Thermo Fischer Scientific Inc., New York, NY, USA). Samples were filtered (0.22 μm PTFE filter membrane, Labex Ltd., Budapest, Hungary) before HPLC analysis and injected onto a Thermo Accucore C18 (100 mm × 2.1, mm i. d., 2.6 μm) column thermostated at 25 °C (±1 °C). The solvents used were water (A) and methanol (B), acidified with 0.1% formic acid, and the flow rate was maintained at 0.2 mL/min. A gradient elution was used: 5% B (0–3 min), a linear gradient increasing from 5% B to 100% (3–43 min), 100% B (43–61 min), a linear gradient decreasing from 100% B to 5% (61–62 min) and 5% B (62–70 min). The column was coupled with a Thermo Q-Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) equipped with an electrospray ionization source. Spectra were recorded in positive and negative-ion mode, respectively. The trace finder 3.1 (Thermo Scientific, USA) software was applied for target screening. Most of the compounds were identified based on previously published studies or data found in the literature. The exact molecular mass, isotopic pattern, characteristic fragment ions, and retention time were always used to identify the molecules.