Ham's F‐12 medium, antibiotics solution, Alexa Fluor 488‐phalloidin, and trypsin‐EDTA were purchased from the Invitrogen Inc. Fetal bovine serum (FBS) was purchased from the Corning Incorporated‐Life Sciences. MTT and DMSO were purchased from the Amresco Inc. Gelatin and Bradford reagent were purchased from the Sigma Chemical Co. Primary antibodies were used at 1:1,000 dilution, except β‐actin (1:10,000). HRP‐conjugated goat anti‐rabbit IgG secondary antibody (Vector Laboratories) was used at 1:5,000 dilution. BS ECL Plus Kit (Biosesang, Inc.) was used to detect protein bands.
Antibiotics solution
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States
Antibiotics solution is a laboratory product that contains a mixture of antibiotics. It is commonly used in cell culture and microbiology applications to prevent bacterial contamination. The solution provides a convenient way to introduce antibiotics into cell culture media or other laboratory samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Merck Group (3 mentions)
Pyruvate, by Thermo Fisher Scientific (2 mentions)
Shiga like toxin 2 stx, by List Biological Laboratories (2 mentions)
14c leucine, by PerkinElmer (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated goat anti rabbit igg secondary antibody, by Vector Laboratories (1 mentions)
Ham s f12 medium, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
β actin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Bs ecl plus kit, by Biosesang (1 mentions)
Tryptose phosphate, by Thermo Fisher Scientific (1 mentions)
M199 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
L glutamic acid, by Merck Group (1 mentions)
Trypsin versene mixture, by Lonza (1 mentions)
Taurine, by Merck Group (1 mentions)
8 protocols using antibiotics solution
1
Cell Culture and Protein Analysis
2
Chicken Embryo Liver Cell Culture for Virus Isolation
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For virus isolation, attenuation and propagation, CEL cell cultures were prepared from livers of 13–15 day-old SPF chicken embryos (VALO Biomedia GmbH, Osterholz-Scharmbeck, Germany) according to a protocol from Schat and Sellers [34 ]. Such cells were also used for virus titration and to establish one-step growth-curves. CEL cells were grown to near confluence in M199 Medium (Invitrogen/Gibco, Paisley, UK) with 10% of fetal bovine serum (FBS), 10% tryptose phosphate and 0.5% of antibiotics solution (all Invitrogen/Gibco) in a controlled atmosphere of 5% CO2 at 37 °C. After infection with the virus and 60–90 min for virus adsorption, the cells were maintained with M199 Medium containing all the additions, except that FBS was reduced to 2%.
3
Cell Culture Reagents and p53 Inhibitor
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l -Glutamic acid, estrogens and their metabolic derivatives, and fetal bovine serum (FBS) are obtained from Sigma (St. Louis, MO). Dullbecco’s modified Eagle’s medium (DMEM) is obtained from Life Technology (Rockville, MD). The antibiotics solution (containing 10,000 U/mL penicillin and 10 mg/mL streptomycin) is obtained from Invitrogen (Carlsbad, CA), and trypsin-versene mixture (containing 0.25% trypsin and 0.02% EDTA) from Lonza Walkersville (Walkersville, MD). Pifithrin-α (PFT-α, p53 inhibitor) is obtained from Calbiochem (San Diego, CA).
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4
Cellular Stress Response Regulation
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Dulbecco's modified Eagle's Ham/F12 medium (DMEM/F12), trypsin/EDTA, fetal bovine serum (FBS), trypsin/EDTA, antibiotics solution, and sterile phosphate buffer saline were purchased from Invitrogen (Carlsbad, CA). Cell culture dishes were obtained from Corning Inc. (Corning, NY). Taurine, epidermal growth factor (EGF), insulin, hydrocortisone, and other chemicals were supplied by Sigma‐Aldrich (St. Louis, MO). Nrf2 small‐interfering RNA (siRNA) and a negative control (NC) siRNA were designed and synthesized by Genepharma (Shanghai, China). p38 MAPK inhibitor (U0126), JNK inhibitor (Sp600125), and ERK1/2 inhibitor (SB203580) were obtained from Sigma‐Aldrich. The following antibodies were employed in this study: anti‐GRP78, anti‐CHOP, anti‐Nrf2, anti‐HO‐1, anti‐NQO‐1, anti‐Xct, and anti‐Txnrd1 antibodies were purchased from Abcam (Cambridge, UK); anti‐JNK, anti‐P‐JNK anti‐ERK, anti‐P‐ERK, anti‐p38, and anti‐P‐p38 antibodies were purchased from Cell Signaling Technology (Beverly, MA); and anti‐β‐actin antibody was obtained from Amyjet Scientific (Wuhan, China).
5
Culturing Human Neuroblastoma Cells
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Human neuroblastoma cells (SH-SY5Y) were Procured from NCCS (National Centre for Cell Sciences, Pune India. Cells were maintained in specific DMEM/F12 medium (Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12) media supplemented with 10% fetal bovine serum (FBS, Thermo) and 1% antibiotics solution (Gibco), and cells were incubated in a humid atmosphere at 37 °C and 5% CO2 incubator.
6
Culturing Glioblastoma Cell Lines
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Human glioma cell lines U251 and U87MG were purchased from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The U87MG cell line was verified through short tandem repeat profiling and demonstrated that the U87MG cells employed in this study corresponded to the U87MG American Type Culture Collection cell line. This particular cell line is classified as a glioblastoma cell line, with undefined origin. Cells were allowed to grow in a medium consisting of RPMI-1640/DMEM (both Hyclone, Logan, UT, USA; Cytiva, Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% antibiotics solution (Gibco; Thermo Fisher Scientific, Inc.). The cells were maintained at 37℃ with 5% CO2. The culture medium was replaced at 72 hours.
7
Shiga-like Toxin 2 Binding Assay
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The following products were purchased from the indicated commercial sources: [ 14 C]-leucine (Perkin-Elmer), Shiga-like toxin 2 (Stx, List Biological Laboratories, Inc.), DMSO (Sigma), fetal bovine serum (Sigma), glutamine, pyruvate, non-essential amino acids and antibiotics solutions (Gibco). Alexa-488-StxB was prepared as previously described. 17
8
Shiga-like Toxin 2 Binding Assay
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The following products were purchased from the indicated commercial sources: [ 14 C]-leucine (Perkin-Elmer), Shiga-like toxin 2 (Stx, List Biological Laboratories, Inc.), DMSO (Sigma), fetal bovine serum (Sigma), glutamine, pyruvate, non-essential amino acids and antibiotics solutions (Gibco). Alexa-488-StxB was prepared as previously described. 17
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