Immunofluorescent analysis was conducted to determine localization of neutrophils and macrophages in the infected wound tissue. Wound tissues were excised and fixed as mentioned above. The tissues were then soaked in PBS containing 30% sucrose and frozen in optimal cutting temperature medium (Sakura Finetek Japan, Tokyo, Japan) at −80 °C. The frozen tissues were cut at 10-μm thickness and the sections were incubated with PBS containing 2% normal goat serum. The sections were stained with rat anti-mouse Ly6G IgG (diluted 1:500; Abcam, United Kingdom) or rat anti-mouse F4/80 IgG (diluted 1:200; Serotec Product, United Kingdom) at 4 °C overnight and then with Alexa 568-conjugated donkey anti-rat IgG (1:200; Invitrogen). The stained sections were observed under BZ-X700 microscope. Ly6G positive cells and F4/80 positive cells were randomly counted from eight histological sections.
Alexa 568 conjugated donkey anti rat igg
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Alexa Fluor 568-conjugated donkey anti-rat IgG is a secondary antibody used for the detection of rat primary antibodies in various immunoassays and imaging techniques. This antibody is conjugated to the Alexa Fluor 568 fluorescent dye, which emits a red fluorescent signal upon excitation.
Automatically generated - may contain errors
Lab products found in correlation
Optimal cutting temperature medium, by Sakura Finetek (1 mentions)
Alexa 488 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Rabbit anti gapdh, by Merck Group (1 mentions)
Rabbit anti olig2, by Merck Group (1 mentions)
Alexa 568 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Mouse anti ng2, by Merck Group (1 mentions)
Alexa 488 conjugated donkey anti goat igg, by Thermo Fisher Scientific (1 mentions)
Mouse anti pan sodium channel, by Merck Group (1 mentions)
Rabbit anti caspr, by Abcam (1 mentions)
Rat anti myelin basic protein mbp, by Bio-Rad (1 mentions)
2 protocols using alexa 568 conjugated donkey anti rat igg
1
Immunofluorescent Analysis of Wound Immune Cells
2
Immunohistochemical Staining for Rodent Myelination
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Primary antibodies used for rodent experiments were rabbit anti-Caspr (Abcam, UK), mouse CC1 monoclonal antibody (Abcam, UK), rabbit anti-GAPDH (Sigma, UK), mouse anti-myelin-associated glycoprotein (MAG, Millipore, UK), rabbit anti-Kv1.1 (Abcam, UK), rat anti-myelin basic protein (MBP, Serotec, UK), chicken anti-neurofilament heavy chain (NFH, Encor Biotechnologies, Florida, United States), rabbit anti-Olig2 (Sigma, UK), mouse anti-O4 (Immunosolv, UK), mouse anti-NG2 (Millipore, UK), mouse anti-proteolipid protein (PLP, Millipore, UK), goat anti-Scribble (C20, Santa Cruz Biotechnology, US), mouse anti-pan sodium channel (Sigma, UK). Secondary antibodies used were Alexa 488-conjugated goat anti-rabbit IgG, Alexa 488-conjugated donkey anti-goat IgG, Alexa 568-conjugated donkey anti-rat IgG, Alexa 568-conjugated donkey anti-mouse IgG, and Alexa 568-conjugated goat anti-rabbit IgG (all from Invitrogen, UK), DyLight 405-conjugated donkey anti-rat IgG, and DyLight 405-conjugated donkey anti-chicken IgG (Stratech, UK).
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