The 3ʹ UTR of human EGR2 or EGR2-Mut amplication was cloned into pGL3-promoter vector. These structures (1 g) were co-transfection with miR-17-5P inhibitor into TPC-1 cells. The luciferase activity was measured at least three times by Dual-luciferase activity analysis system (Promega) at 48 h after transfection.
Dual luciferase activity analysis system
Manufactured by Promega
The Dual-luciferase activity analysis system is a laboratory equipment designed for the quantitative measurement of two different luciferase reporter enzymes within the same sample. It provides a rapid and sensitive method for analyzing gene expression and signal transduction pathways.
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Lab products found in correlation
2 protocols using dual luciferase activity analysis system
1
Regulation of EGR2 by miR-17-5P
2
Lnc MST1P2 and miR-133b Interaction
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Co-transfected Lnc MST1P2 and miR-133b, NC-inh, miR-133b-inh, with pmirGlo-NC, pmirGlo-Lnc MST1P2-mut or pmirGloLnc MST1P2-wt into HeLa and SIHA cells. The luciferase activity was measured at least three times by Dual-luciferase activity analysis system (Promega) at 48 hours after transfection.
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