Interferon β ifnβ

Manufactured by Thermo Fisher Scientific

Interferon β (IFNβ) is a laboratory reagent produced by Thermo Fisher Scientific. It is a type I interferon protein that plays a role in the body's immune response. IFNβ functions as a signaling molecule, activating cellular pathways involved in anti-viral and anti-proliferative activities.

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Lab products found in correlation

Mem eagle, by PAN Biotech (1 mentions) Penicillin streptomycin, by PAN Biotech (1 mentions) Fungizone, by PAN Biotech (1 mentions) L glutamine, by Harvard Bioscience (1 mentions) Il 18bp fc, by Thermo Fisher Scientific (1 mentions) Ifn λ1, by Thermo Fisher Scientific (1 mentions) Chondroitin sulfate b, by Merck Group (1 mentions) Tnf β, by Thermo Fisher Scientific (1 mentions) Ifn ω, by Thermo Fisher Scientific (1 mentions) Ifn γ, by Thermo Fisher Scientific (1 mentions)

2 protocols using interferon β ifnβ

Culturing and Stimulating Human Synovial Fibroblasts

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Human synovial fibroblasts (HSF) were obtained from ScienCell Research Laboratory (ScienCell, 4700; Clinisciences). The primary cultures of HSF were maintained in Minimum Essential Medium eagle (MEM eagle, PAN Biotech P0408500) supplemented with 10% of decomplemented fetal bovine serum (PAN Biotech, 3302 P290907) and completed with L-glutamine 2 mM (Biochrom AG, K0282), 100U/mL– 0.1 mg/mL penicillin- streptomycin (PAN Biotech, P0607100), 1 mM sodium pyruvate (PAN Biotech, P0443100) and 0.5 μg/mL fungizone (PAN Biotech, P0601001).
The double-stranded polyribonucleotide PIC (cat. no.27-4732-01) was purchased from Amersham Biosciences. Interleukin-1β (IL-1β; cat. no. 200-01B), Tumor necrosis factor-α (TNFα; cat. no. 300-01A) and Interferon β (IFNβ; cat. no. 300-02BC) were purchased from Peprotech.
A clinical isolate (clone CHIKV 4.2) amplified from a CHIKV-infected serum sample (isolated during the 2006 epidemic) was used after two passages on Vero cells [8 (link)].

Bedoui Y., Septembre-Malaterre A., Giry C., Jaffar-Bandjee M.C., Selambarom J., Guiraud P, & Gasque P. (2021). Robust COX-2-mediated prostaglandin response may drive arthralgia and bone destruction in patients with chronic inflammation post-chikungunya. PLoS Neglected Tropical Diseases, 15(2), e0009115.

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Cytokine Library Reconstitution and Characterization

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Recombinant human cytokines used in this study [CCL1, CCL2, CCL3, CCL3L1, CCL4, CCL4L1, CCL5, CCL7, CCL8, CCL11, CCL13, CCL14, CCL15, CCL16, CCL17, CCL18, CCL19, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, CXCL10, CXCL11, CXCL12α, CXCL12β, CXCL13, CXCL14, CXCL16, XCL1, CX3CL1, interleukin-1α (IL-1α), IL-1β, IL-6, IL-6Rα, IL-10, IL-12p70, IL-13, IL-17a, IL-18BP-Fc, IL-23, IL-27, IL-35, tumor necrosis factor–α (TNF-α), TNF-β, interferon-β (IFN-β), IFN-γ, IFN-λ1, and IFN-ω] from PeproTech and IFN-α2 and IL-18 from Sino Biological were reconstituted in DPBS 0.1% BSA at 10 μM, aliquoted, and stored at −80°C. Heparin (no. 2106), heparan sulfate from bovine kidney (no. H7640), chondroitin sulfate A (no. C9819), and chondroitin sulfate B (no. C3788) were obtained from MilliporeSigma. Heparan sulfate from porcine mucosa (no. AMS.GAG-HS01) and keratan sulfate (no. AMS.CSR-NAKPS2-SHC-1) were purchased from AMSBIO. We assumed an average molecular weight of 30 kDa for heparan sulfate from porcine mucosa and 15 kDa for heparin (37 ).

López-Muñoz A.D., Kosik I., Holly J, & Yewdell J.W. (2022). Cell surface SARS-CoV-2 nucleocapsid protein modulates innate and adaptive immunity. Science Advances, 8(31), eabp9770.

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