Log In Sign up
The largest database of trusted experimental protocols

Tnfα pe clone mp6 xt22

Manufactured by BD
Visit Supplier

The TNFα-PE (clone MP6-XT22) is a flow cytometry reagent that detects the presence of tumor necrosis factor alpha (TNFα) in biological samples. It consists of an anti-TNFα monoclonal antibody conjugated to the fluorescent dye R-Phycoerythrin (PE). This reagent can be used to identify and quantify TNFα-expressing cells in various cell types and experimental conditions.

Automatically generated - may contain errors

Lab products found in correlation

Cytofix cytoperm kit, by BD (2 mentions) 2.4g2 antibody, by BD (1 mentions) Cd11c apc clone hl3, by BD (1 mentions)

Spelling variants of this product

TNFα (MP6-XT22; (10 citations) MP6-XT22 (9 citations) TNFα (clone MP6-XT22) (6 citations) Clone MP6-XT22) (3 citations) TNFα-PE (MP6-XT22, (2 citations) PE TNFα (2 citations)

2 protocols using tnfα pe clone mp6 xt22

1

Quantifying Antigen-Specific T Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
2×106 cells from single-cell suspensions were incubated at 37°C in RPMI 1640 complete media containing 2% FBS, 1μg/ml brefeldin A and 1μM Site I peptide (206SAINNYAQKL215) or H-2Kb/HSV glycoprotein B (gB) peptide (498SSIEFARL505). Following a 4-hour incubation, cells were stained for CD8 and CD90.1. The Cytofix/Cytoperm kit (BD Pharmingen) was used to permeabilize and intracellularly stain cells with IFNγ-FITC (clone XMG1.2) and TNFα-PE (clone MP6-XT22) antibodies per the manufacturer’s instructions. Site I-reactive T cells was calculated by subtracting the proportion of gB peptide responders in parallel samples.
Ward-Kavanagh L.K., Kokolus K.M., Cooper T.K., Lukacher A.E, & Schell T.D. (2018). Combined sublethal irradiation and agonist anti-CD40 enhance donor T cell accumulation and control of autochthonous murine pancreatic tumors. Cancer immunology, immunotherapy : CII, 67(4), 639-652.
+ Open protocol
+ Expand
2

Multiparameter Identification of Monocyte Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Markers used to identify monocyte/macrophage subsets included: F4/80-PerCP Cy5.5 or APC (clone BM8; eBioscience & Biolegend), Ly6C-FITC, PE or APC-Cy7 (clone AL-21; BD Biosciences), CCR2-PE or APC (Clone 475301; R&D Systems), MHC class II-PE (clone M5/114.15.2; BD Biosciences), CD11c-APC (clone HL3; BD Biosciences), CD206-PE (C-type Mannose Receptor 1, clone MR5D3; AbD Serotec), CD14-PE (clone Sa2–8; eBioscience), MerTK-APC (clone 108928; R&D Systems) and CD64-PE (clone X54-5/7.1.1; BD Biosciences). Surface FcγRII/III was blocked with 2.4G2 antibody (BD Biosciences). Cells were surface-stained for 30 minutes on ice in staining buffer (1x DPBS/1% FCS). Where indicated, cells were fixed for 30 minutes, treated with 1x permeabilization wash (Cytofix/Cytoperm Kit; BD Biosciences) and intracellularly stained with TNF-α-PE (clone MP6-XT22; BD Biosciences).
Crane M.J., Daley J.M., van Houtte O., Brancato S.K., Henry WL J.r, & Albina J.E. (2014). The Monocyte to Macrophage Transition in the Murine Sterile Wound. PLoS ONE, 9(1), e86660.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!