Twelve rats were randomly divided into four groups: sham group and SCI group (1 day, 2 days, and 3 days after SCI) (n = 3 per group). The parenchymal tissue at the surgery site was extracted for western blotting and qRT-PCR analysis, as previously described. The remaining 48 rats were randomly divided into the following four groups, and testing was performed by blinded observers: (1) sham group (the rats were subjected to sham operation), (2) control group (the rats were subjected to SCI and were treated with PBS), (3) agomir-NC group (the rats were subjected to SCI and treated with agomir-NC [RiboBio, Guangzhou, China]), and (4) agomir-135a-5p group (the rats were subjected to SCI and treated with 25 μL of 10 nmol agomir-135a-5p [RiboBio, Guangzhou, China]) (n = 12 per group). The damaged spinal cords were collected 7 days after intrathecal injection for further western blotting and immunofluorescence analyses.
Wang N., Yang Y., Pang M., Du C., Chen Y., Li S., Tian Z., Feng F., Wang Y., Chen Z., Liu B, & Rong L. (2020). MicroRNA-135a-5p Promotes the Functional Recovery of Spinal Cord Injury by Targeting SP1 and ROCK. Molecular Therapy. Nucleic Acids, 22, 1063-1077.
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