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Alexa fluor 647 conjugated goat anti human igg secondary antibody

Manufactured by Thermo Fisher Scientific
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Alexa Fluor 647 conjugated goat anti-human IgG secondary antibody is a fluorescently labeled antibody that binds to human immunoglobulin G (IgG) antibodies. The Alexa Fluor 647 dye allows for detection and visualization of target IgG proteins in various applications.

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Lab products found in correlation

Human cd80 fc, by R&D Systems (2 mentions) Mouse anti human cd28 clone 28.2, by Thermo Fisher Scientific (2 mentions) Human cd86 fc, by R&D Systems (2 mentions) 293t crl 3216, by American Type Culture Collection (2 mentions) Jurkat e6.1 t cells, by American Type Culture Collection (2 mentions)

Close spelling variants of this product

Goat anti-human IgG-Alexa Fluor® 647 secondary antibody Alexa-Fluor-647-conjugated goat anti-human IgG Alexa Fluor 647 goat anti-human IgG antibody Alexa Fluor 647-conjugated goat anti-human antibody Alexa Fluor 647 goat anti-human IgG Alexa Fluor 647-conjugated secondary antibodies Alexa Fluor 647 anti-goat IgG Alexa Fluor 647 anti-human IgG Alexa Fluor 647 secondary antibody Alexa Fluor 647-conjugated antibody

2 protocols using alexa fluor 647 conjugated goat anti human igg secondary antibody

1

CD28 Receptor Binding Kinetics

Check if the same lab product or an alternative is used in the 5 most similar protocols
293T (CRL-3216) and Jurkat T cells (E6.1) were obtained from ATCC (American Type Culture Collection). They were routinely checked for mycoplasma and were mycoplasma free. transduced with pLenti-TOPO containing wild-type CD28 and the CD28 mutants. CD28 expression was detected with a mouse anti-human CD28 (Clone 28.2; dilution 1:100; eBioscience). The binding of human CD80-Fc (catalog number 140-B1-100; R&D Systems) and human CD86-Fc (catalog number 141-B2-100; R&D Systems) to these cells were performed as previously described29 (link),58 (link). The secondary antibody used was Alexa Fluor 647 conjugated goat anti-human IgG secondary antibody (catalog number A-21445; concentration: 5 μg/ml; Life Technologies). Kd were calculated based on the presumption of one binding site. Kd= [A][B]/[AB] where in A represent soluble CD80-Fc or Cd86-Fc and B represents CD28 molecules on the cell surface of 293T cells.
Choi J., Goh G., Walradt T., Hong B.S., Bunick C.G., Chen K., Bjornson R.D., Maman Y., Wang T., Tordoff J., Carlson K., Overton J.D., Liu K.J., Lewis J.M., Devine L., Barbarotta L., Foss F.M., Subtil A., Vonderheid E.C., Edelson R.L., Schatz D.G., Boggon T.J., Girardi M, & Lifton R.P. (2015). Genomic landscape of cutaneous T cell lymphoma. Nature genetics, 47(9), 1011-1019.
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2

CD28 Receptor Binding Kinetics

Check if the same lab product or an alternative is used in the 5 most similar protocols
293T (CRL-3216) and Jurkat T cells (E6.1) were obtained from ATCC (American Type Culture Collection). They were routinely checked for mycoplasma and were mycoplasma free. transduced with pLenti-TOPO containing wild-type CD28 and the CD28 mutants. CD28 expression was detected with a mouse anti-human CD28 (Clone 28.2; dilution 1:100; eBioscience). The binding of human CD80-Fc (catalog number 140-B1-100; R&D Systems) and human CD86-Fc (catalog number 141-B2-100; R&D Systems) to these cells were performed as previously described29 (link),58 (link). The secondary antibody used was Alexa Fluor 647 conjugated goat anti-human IgG secondary antibody (catalog number A-21445; concentration: 5 μg/ml; Life Technologies). Kd were calculated based on the presumption of one binding site. Kd= [A][B]/[AB] where in A represent soluble CD80-Fc or Cd86-Fc and B represents CD28 molecules on the cell surface of 293T cells.
Choi J., Goh G., Walradt T., Hong B.S., Bunick C.G., Chen K., Bjornson R.D., Maman Y., Wang T., Tordoff J., Carlson K., Overton J.D., Liu K.J., Lewis J.M., Devine L., Barbarotta L., Foss F.M., Subtil A., Vonderheid E.C., Edelson R.L., Schatz D.G., Boggon T.J., Girardi M, & Lifton R.P. (2015). Genomic landscape of cutaneous T cell lymphoma. Nature genetics, 47(9), 1011-1019.
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