Huh7, Hep3B, PLC/PRF/5 and HepG2 cells (human HCC lines) were obtained from the Korean Cell Line Bank. Human HCC cell line Huh6 was kindly provided by Dr. Ralf Bartenschlager (University of Heidelberg, Germany) and Fa2N-4 cells (human immortalized hepatocyte cell line) were purchased from Xenotech (Lenexa, KS, USA). HCC cell lines were cultured in Dulbecco’s minimal essential medium (DMEM; Welgene, Korea, LM001-05) supplemented with heat-inactivated 10% fetal bovine serum (FBS; Gibco, Gaitherburg, MD, USA) and 100U/ml Penicillin and 100 μg/ml Streptomycin (Gibco) at humidified 37 °C incubator under 5% CO2. Fa2N-4 cells were plated in collagen-coated plates. After cell attachment (approximately 3 ~ 6 h), serum-containing plating medium (XenoTech, K4000) was replaced with supporting culture medium (XenoTech, K4100.X).
Lm001 05
Manufactured by Thermo Fisher Scientific
Sourced in United States
The LM001-05 is a laboratory equipment product from Thermo Fisher Scientific. It is a precision instrument designed for accurate measurement and analysis in a laboratory setting. The core function of the LM001-05 is to provide reliable and consistent performance for laboratory professionals.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Plc prf 5, by Korean Cell Line Bank (1 mentions)
Hep3b, by Korean Cell Line Bank (1 mentions)
Fa2n 4 cells, by Xenotech (1 mentions)
K4000, by Xenotech (1 mentions)
Hepg2, by Korean Cell Line Bank (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
2 protocols using lm001 05
1
Culturing Human Hepatocellular Carcinoma Cell Lines
2
Culturing HEK293T, HepG2, and HeLa Cells
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HEK293T (ATCC, CRL-11268), HepG2 (ATCC, HB-8065) and HeLa (ATCC, CCL-2) cells were cultured in Dulbecco’s Modified Eagle Medium (Welgene, LM001-05) supplemented with 10% fetal bovine serum (Gibco, 16000) and an antibiotic-antimycotic (Gibco, 15240) in a humidified atmosphere containing 5% CO2 at 37°C.
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