Rabbit anti dtak1

The following primary antibodies were used for Western blot and immunostaining assays: Mouse anti-Flag (1:5000, Sigma-Aldrich, Cat#F1804); Rabbit anti-Myc (1:3000, Medical & Biological Laboratories, Cat#562); Mouse anti-dTrbd (1:1000), which was generated to an N-terminal fragment of dTrbd (amino acids 155-253) fused to His and purified from E. coli; Rabbit anti-dTak1 (1:1000, Abcam, Cat#239353); Mouse anti-β-Tubulin (1:3000, Cowin, Cat#CW0098M); Rabbit anti-HA (1:2500, Medical & Biological Laboratories, Cat#561); Mouse anti-GFP (1:2000, Sigma-Aldrich, Cat#G6539); Mouse anti-V5 (1:5000, Sigma-Aldrich, Cat#V8012).
The secondary antibodies for Western blot and immunostaining assays include Goat anti-Mouse IgG H&L (HRP); Goat anti-Rabbit IgG H&L (HRP); Goat anti-Mouse IgG H&L (Alexa Fluor 555) (1:1000, Abcam, Cat#ab150078).

The following primary antibodies were utilized for Western blot assays: Rabbit anti-Myc (1:3000, Medical & Biological Laboratories, Cat#562); Mouse anti-β-Tubulin (1:3000, Cowin, Cat#CW0098M); Mouse anti-Flag (1:3000, Sigma-Aldrich, Cat#F1804); Rabbit anti-HA (1:3000, Medical & Biological Laboratories, Cat#561); Mouse anti-Puromycin (1:5000, Sigma-Aldrich, Cat#MABE343); Rabbit anti-dTak1 (1:1000, Abcam, Cat#239353) (Tsapras et al, 2022 (link)); Mouse anti-Ubiquitin (1:1000, Santa Cruz, Cat#sc-8017); Rabbit anti-UbK48 (1:1000, Sigma-Aldrich, Cat#SAB5701119); Mouse anti-eIF3f1 (1:2000), which was generated by immunizing mice with the purified N-terminal fragment of eIF3f1 (amino acids from 41 to 140).
The secondary antibodies utilized in this study include Goat anti-mouse IgG H&L (1:5000, Abcam, Cat#ab150078) and Goat anti-rabbit IgG H&L (1:5000, Abcam, Cat#ab6789).