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Chicken anti rat alexafluor 488 secondary antibody

Manufactured by Thermo Fisher Scientific
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The Chicken-anti-rat AlexaFluor 488 secondary antibody is a fluorescently labeled antibody that binds to rat primary antibodies. The AlexaFluor 488 dye provides green fluorescence when excited at the appropriate wavelength, enabling visualization and detection of target molecules in biological samples.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (2 mentions)

Close spelling variants of this product

Anti-rat AlexaFluor 488 secondary antibody Anti Alexafluor-488 antibody 488 anti-chicken AlexaFluor antibody

2 protocols using chicken anti rat alexafluor 488 secondary antibody

1

Quantifying CCR10 Expression in hPSCs

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HPSCs were grown to 90% confluency, lifted with enzyme-free dissociation buffer and washed with PBS. Cells were counted and 1×106 cells aliquoted in 1% (w/v) BSA/PBS. From here on all manipulations were on ice or at 4°C. Cells were incubated for 1h with CCR10 antibody (MAB3478 R&D Systems) or the isotype control (rat IgG, eBiosciences, San Diego, CA). Cells were washed with BSA/PBS prior to and immediately after incubation with a chicken-anti-rat AlexaFluor 488 secondary antibody (Life Technologies, Madison WI). The cells were fixed in 1% (w/v) PFA in PBS and cell immunostaining data acquired using a BD-LSRII flow cytometer. Data was analyzed used FlowJo software (FLOWJO LLC, Ashland OR).
Roy I., Boyle K.A., Vonderhaar E.P., Zimmerman N.P., Gorse E., Mackinnon A.C., Hwang R., Franco-Barraza J., Cukierman E., Tsai S., Evans D.B, & Dwinell M.B. (2017). Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma. Laboratory investigation; a journal of technical methods and pathology, 97(3), 302-317.
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2

Quantifying CCR10 Expression in hPSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
HPSCs were grown to 90% confluency, lifted with enzyme-free dissociation buffer and washed with PBS. Cells were counted and 1×106 cells aliquoted in 1% (w/v) BSA/PBS. From here on all manipulations were on ice or at 4°C. Cells were incubated for 1h with CCR10 antibody (MAB3478 R&D Systems) or the isotype control (rat IgG, eBiosciences, San Diego, CA). Cells were washed with BSA/PBS prior to and immediately after incubation with a chicken-anti-rat AlexaFluor 488 secondary antibody (Life Technologies, Madison WI). The cells were fixed in 1% (w/v) PFA in PBS and cell immunostaining data acquired using a BD-LSRII flow cytometer. Data was analyzed used FlowJo software (FLOWJO LLC, Ashland OR).
Roy I., Boyle K.A., Vonderhaar E.P., Zimmerman N.P., Gorse E., Mackinnon A.C., Hwang R., Franco-Barraza J., Cukierman E., Tsai S., Evans D.B, & Dwinell M.B. (2017). Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma. Laboratory investigation; a journal of technical methods and pathology, 97(3), 302-317.
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