Gb12139

Manufactured by Wuhan Servicebio Technology

Sourced in China

The GB12139 is a laboratory equipment product. It is designed for specific functions within a laboratory setting. No further details about its core function or intended use can be provided in a factual and unbiased manner.

Automatically generated - may contain errors

Lab products found in correlation

Gb27303, by Wuhan Servicebio Technology (1 mentions) Gb25301, by Wuhan Servicebio Technology (1 mentions) Gb113373, by Wuhan Servicebio Technology (1 mentions) Ap0564, by ABclonal (1 mentions) Gb11063, by Wuhan Servicebio Technology (1 mentions) Anti neun antibody, by Wuhan Servicebio Technology (1 mentions) Gb11119, by Wuhan Servicebio Technology (1 mentions) Gb11096, by Wuhan Servicebio Technology (1 mentions) Gb11285, by Wuhan Servicebio Technology (1 mentions)

2 protocols using gb12139

Immunofluorescent Analysis of Rat Femur Fracture

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Rat femur fracture specimens from six-week-old SD rats were obtained at 14 d, fixed for 24 h in 4% paraformaldehyde, embedded in paraffin, and then sectioned for histological analysis. The manufacturer's instructions were followed for staining various immunofluorescent proteins. The S100β (1:1000, GB12359; Servicebio, Wuhan, China) and β III-tubulin (1:1000, GB12139; Servicebio, Wuhan, China) specific primary antibodies were used in this work. Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (1:400, GB25301, Servicebio, Wuhan, China) or cy5-conjugated goat anti-rabbit IgG (GB27303, Servicebio, Wuhan, China) was used as the secondary antibody and incubated at 25 °C for 2 h before being combined with DAPI for co-incubation at 25 °C.

Wang T., Li W., Zhang Y., Xu X., Qiang L., Miao W., Yue X., Jiao X., Zhou X., Ma Z., Li S., Ding M., Zhu J., Yang C., Wang H., Li T., Sun X, & Wang J. (2023). Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair. Bioactive Materials, 27, 377-393.

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Spinal Cord Injury Histology

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Euthanasia of rats by 2% sodium pentobarbital injection followed by intracardiac perfusion fixation with PBS and 4% paraformaldehyde. The spinal cord was embedded in paraffin and then cut into 20 µm thick sections using electric slicer (Leica, Germany). HE and immunofluorescence staining using primary antibody including anti‐iNOS antibody (GB11119, Servicebio, China), anti‐F4/80 antibody (GB113373, Servicebio, China), anti‐ARG1 antibody (GB11285, Servicebio, China), anti‐CD31 antibody (GB11063, Servicebio, China), anti‐Tuj‐1 antibody (GB12139, Servicebio, China), anti‐GFAP antibody (GB11096, Servicebio, China), anti‐GAP43 antibody (GB11095, Servicebio, China), anti‐NG2 antibody (GB115534, Servicebio, China), anti‐TOMM20 antibody (GB111481, Servicebio, China), anti‐p‐P70S6K antibody (AP0564, Abclonal, China), anti‐NeuN antibody (Servicebio, China) in samples were used to evaluate lesion cavity and nerve regeneration.

Jiang X., Wang W., Tang J., Han M., Xu Y., Zhang L., Wu J., Huang Y., Ding Z., Sun H., Xi K., Gu Y, & Chen L. (2023). Ligand‐Screened Cerium‐Based MOF Microcapsules Promote Nerve Regeneration via Mitochondrial Energy Supply. Advanced Science, 11(6), 2306780.

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