Alexa fluor 488 or 647 conjugated secondary antibodies

Manufactured by Jackson ImmunoResearch

Sourced in United States

Alexa Fluor® 488 or 647 conjugated secondary antibodies are fluorescent-labeled detection reagents used in various immunodetection techniques, such as Western blotting, immunofluorescence, and flow cytometry. These antibodies are designed to bind to the primary antibody, allowing for the visualization and quantification of target proteins or cellular components.

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Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti avl9, by Abcam (1 mentions) Anti γ tubulin, by Abcam (1 mentions) Anti e cadherin, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Prolong diamond antifade mounting medium, by Thermo Fisher Scientific (1 mentions) Troma 1, by Developmental Studies Hybridoma Bank (1 mentions)

2 protocols using alexa fluor 488 or 647 conjugated secondary antibodies

Immunofluorescence Staining Protocol

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Antibodies used include anti-E-cadherin (Santa Cruz, Dallas, Texas, USA), anti-α-tubulin (DM1A clone from Sigma, Saint Louis, MO, USA), anti-γ-tubulin (Abcam, Cambridge, MA), anti-cleaved caspase3 (Cell Signaling, Danvers, MA, USA), anti-NUPL1 (Sigma), anti-DENND5A (Abcam), and anti-AVL9 (Abcam). Alexa Fluor^® 488 or 647 conjugated secondary antibodies are from Jackson Immunoresearch, West Grove, PA, USA. Rhodamine phalloidin for F-actin-staining and DAPI (1:1000) for nucleus-staining are from Invitrogen, Grand Island, NY, USA.

Li Y., Xu J., Xiong H., Ma Z., Wang Z., Kipreos E.T., Dalton S, & Zhao S. (2014). Cancer driver candidate genes AVL9, DENND5A and NUPL1 contribute to MDCK cystogenesis. Oncoscience, 1(12), 854-865.

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Immunostaining of Frozen Tissue Sections

Cited 1 time

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Frozen tissues were sectioned at 10 μm thickness and placed onto charged slides for immunostaining as previously described [11 (link)]. Primary antibodies against E-cadherin (1:500, R&D systems, AF 748) and Keratin (K) 8 (1:500, Developmental Studies Hybridoma Bank, TROMA-1) were used. Alexa Fluor 488- or 647-conjugated secondary antibodies (1:500, Jackson Immunoresearch) were utilized for visualizations. Slides were mounted with Prolong® Diamond antifade mounting medium (Fisher Scientific, P36970).

Kramer N., Chen G., Ishan M., Cui X, & Liu H.X. (2019). Early taste buds are from Shh+ epithelial cells of tongue primordium in distinction from mature taste bud cells which arise from surrounding tissue compartments. Biochemical and biophysical research communications, 515(1), 149-155.

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