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Omcl ac240ts c3

Manufactured by Olympus
Sourced in Japan

The OMCL-AC240TS-C3 is a lab equipment product from Olympus. It is a high-precision autoclave with a capacity of 240 liters and a temperature range of up to 134°C. The equipment is designed for sterilization of medical and laboratory instruments.

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2 protocols using omcl ac240ts c3

1

Determination of Bulk Gel Elasticity

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To
determine the value of E of bulk gels, we also
used an atomic force microscope (MFP-3D; Asylum Research) equipped
with a tetrahedral cantilever (OMCL-AC240TS-C3; Olympus, Japan). The E value of the gels was determined by deforming the surface
and fitting the force–indentation curve to the Hertz (Sneddon’s
variation) model:33 (link) Here, F is the applied force
calculated using κ, and δ the indentation length according
to the difference between the piezo height and cantilever deflection.
The elastic modulus (E) and Poisson’s ratio
(ν) together define the material properties. Poisson’s
ratio ν was assumed to be 0.5 because the gelatin gel was considered
incompressible, as has been previously reported.34 (link) The cantilever properties were defined by the opening angle
α (=34°) and cantilever spring constant κ. A thermal
vibration based program installed in the AFM instrument was used to
calculate κ. The cantilever was calibrated before each experiment,
and the average κ for the experiments was 2 N/m. By analyzing
the approach curve, we obtained E.
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2

Atomic Force Microscopy of Protoplast Fibers

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Fibers secreted from protoplasts were observed by atomic force microscopy (AFM). Samples for AFM were prepared by washing with 50 mM methylenediaminetetraacetic acid (pH 7.0) and distilled water in three times, respectively. For observation, they were put on a microscope slide and then air-dried. Atomic force microscopy images for surfaces of the dried sample were acquired with a MFP-3D-SA (Oxford Instruments Asylum Research, Inc., California, USA) operated at room temperature, being controlled in the non-contact mode with a scan rate 1.0 Hz to observe 15 × 15 µm 2 areas. The AFM tip employed was an etched-silicon AFM tip (OMCL-AC240TS-C3, Olympus, Tokyo, Japan) with a nominal radius from to 7 nm, and a spring (force) constant of 2 N m -1 .
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