Anti il 2 pecy7

Manufactured by BD

Anti-IL-2-PEcy7 is a monoclonal antibody conjugated with the fluorochrome Phycoerythrin-cyanine 7 (PE-Cy7). It is designed to detect interleukin-2 (IL-2), a cytokine involved in immune cell activation and proliferation. This product can be used in flow cytometry applications to identify and analyze IL-2-producing cells.

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2 protocols using anti il 2 pecy7

Multiparametric Flow Cytometry Analysis of T Cell Responses

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The fluorochrome labeled monoclonal antibodies (mAbs) anti-CD3-Alexa 700, anti-CD4-V450, anti-CD4-PEcy7, anti-CD8-APC, anti-CD8-V450, anti-CD2-APC, anti-CD28-FITC, anti-TNF-α-PE, anti-IFN-γ-PerCPcy5.5, and anti-IL-2-PEcy7 were purchased from BD Biosciences (Franklin Lakes, NJ). Anti-CD45RA-QDOT655 mAb and carboxyfluorescein succinimidyl ester (CFSE) were obtained from Invitrogen (Carlsbad, CA). Anti-CD197-APC anti-CD8-Alexa780 mAb was obtained from Ebioscience (San Diego, CA). Human CMV peptide pool of pp65 sequence consisting of 138 peptides (15 mers with 11 amino acid overlaps) was purchased from JPT Peptide Technologies (Berlin, Germany). Perm/Wash buffer, Cytofix/Cytoperm solution, and GolgiPlug containing brefeldin A were obtained from BD Biosciences.

Xu H., Perez S.D., Cheeseman J., Mehta A.K, & Kirk A.D. (2014). The allo- and viral-specific immunosuppressive effect of belatacept, but not tacrolimus, attenuates with progressive T cell maturation. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 14(2), 319-332.

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Multi-parameter Flow Cytometry of Immune and Stromal Cells

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PBMCs and SFMCs were stained for flow cytometry using anti‐CD4 APC (clone: RPA‐T4, BioLegend, 300 552), anti‐CD8 AF700 (clone: RPA‐T8, BD, 56–0088‐41), anti‐CD25 PerCP‐Cy5.5 (clone: M‐A251, BD, 560503), anti‐PD‐1 AF488 (clone: EH12.2H7, BioLegend, 329 936), anti‐Gal‐3 PE (clone: Gal397, BD, 126706), anti‐IL‐2 PECy7 (clone: MQ1 17H12, BD, 560707) and nIR Live/Dead marker (Thermo Fisher, L34976), all at recommended concentrations. For intracellular staining the True‐Nuclear™ Transcription Factor Buffer Set (Biolegend, 424 401) was used.
FLS were stained for flow cytometry using anti‐CD90 BV605 (clone: 5E10, BioLegend, 328 128), anti‐ICAM BV421 (clone: HA58, BD, 564077) and anti‐PD‐L1 PECy7 (clone: MIH3, BioLegend, 374 506) as well as intracellular: anti‐Gal‐3 PECF594 (clone: B2C10, BD, 565682).
Flow cytometric data acquisition was performed using the NovoCyte Quanteon® (ACEA Bioscience, San Diego, CA, USA) and flow cytometric analysis were performed in FlowJo™ 10.7.1. Gating was performed by including live, single cells. Additionally, gating was performed using fluorescence minus one (FMO) control (Figure S1, Figure S3+c).

Pedersen K., Nielsen M.A., Juul‐Madsen K., Hvid M., Deleuran B, & Greisen S.R. (2022). Galectin‐3 interacts with PD‐1 and counteracts the PD‐1 pathway‐driven regulation of T cell and osteoclast activity in Rheumatoid Arthritis. Scandinavian Journal of Immunology, 97(2), e13245.

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