Protein carbonyls were measured in heart lysates using the Protein Carbonyl ELISA kit (Cell BioLabs, Inc, San Diego, CA). Hydrogen peroxide was measured in frozen heart lysates using the Amplex Red Assay kit (Invitrogen, Grand Island, NY). Aconitase activity was measured in frozen heart lysates using a commercially available kit (Cayman Chemical, Ann Arbor, MI). Western blotting was performed on heart lysates. Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes and probed with the following antibodies: anti-ACC (Cell Signaling Technology, Danvers, MA), anti-sarcomeric actin (Sigma Aldrich, St. Louis, MO).
Anti sarcomeric actin
Manufactured by Merck Group
Anti-sarcomeric actin is a laboratory reagent used in research applications. It is an antibody that specifically binds to sarcomeric actin, which is a key structural protein found in muscle cells. This product can be used to detect and visualize the presence of sarcomeric actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti acc, by Cell Signaling Technology (1 mentions)
Amplex red assay kit, by Thermo Fisher Scientific (1 mentions)
Protein carbonyl elisa kit, by Cell Biolabs (1 mentions)
Isoproterenol, by Merck Group (1 mentions)
Anti fn1, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Anti actb, by Santa Cruz Biotechnology (1 mentions)
Anti smad4, by Cell Signaling Technology (1 mentions)
Anti col1a1, by Santa Cruz Biotechnology (1 mentions)
Anti smad3, by Cell Signaling Technology (1 mentions)
Protocatechuic acid, by Merck Group (1 mentions)
2 protocols using anti sarcomeric actin
1
Oxidative Stress Biomarkers in Heart
2
Investigating Cardiac Fibrosis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protocatechuic acid (cat no. 37580, lot no. BCCF0948), isoproterenol (cat no. I5627, lot no. BCCC9596) and anti‐sarcomeric actin (cat no. A2172, lot no. 128M4892V) antibody were obtained from Sigma‐Aldrich. Anti‐Actb (cat no. sc‐47778, lot no. K2222), anti‐Fn1 (cat no. sc‐59826, lot no. F1814), anti‐Col1a1 (cat no. sc‐293182, lot no. H2619) and anti‐BNP (Nppb, sc‐271185, lot no. L3119) antibodies were purchased from Santa Cruz Biotechnology. Anti‐Kmo antibodies (cat no. MAB8050‐100, lot no. CMPW0119101) were purchased from Bio‐Techne. Alexa Fluor 488 phalloidin (cat no. A12379, lot no. 2129460) was obtained from Invitrogen. Anti‐Col 3 (ab7778) antibody was purchased from Abcam. Anti‐Smad3 (9523T, lot no. 7) and anti‐Smad4 (3845T, lot no. 3) were purchased from Cell Signaling.
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