Larval movement in response to insecticide exposure was quantified by Wiggle Index Assay, as described by Denecke et al., 2015 (link). 25 third-instar larvae were used for a single biological replicate and 4 replicates were tested for each exposure condition. Undosed larvae in NUNC cell plates (Thermo Scientific) in 5% Analytical Reagent Sucrose (Chem Supply) solution were filmed for 30 s, and then 30 min, 1 hr, 1 hr, and 30 min and 2 hr after spinosad exposure. The motility at each time point is expressed in terms of relative movement ratio (RMR), normalized to motility prior to spinosad addition.
Nunc cell plates
Manufactured by Thermo Fisher Scientific
The NUNC cell plates are a line of cell culture plates designed for the growth and maintenance of cells in a laboratory setting. These plates provide a standardized and consistent surface for cell adhesion and proliferation, enabling researchers to conduct reliable and reproducible cell-based experiments.
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Lab products found in correlation
2 protocols using nunc cell plates
1
Larval Motility Response to Insecticide
2
Larval Motility Assay for Insecticide
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Larvae movement in response to insecticide exposure was quantified by Wiggle Index Assay, as described by Denecke et al. (2015) (link). 25 third instar larvae were used for a single biological replicate and four replicates were tested for each exposure condition. Undosed larvae in NUNC cell plates (Thermo-Scientific) in 5% Analytical Reagent Sucrose (Chem Supply) solution were filmed for 30 seconds and then 30 min, 1 hr, 1 hr and 30 min and 2 hr after spinosad exposure. The motility at each time-point is expressed in terms of Relative Movement Ratio (RMR), normalized to motility prior to spinosad addition.
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