Milli qa10 filtration system

The Ti6Al4V powder was purchased from AK Medical Co., Ltd. (Beijing, China). Poloxamer 407 powder was obtained from Bayee Chemical Co., Ltd. (Hangzhou, China). BMP-2, OPG, and antibodies used in immunofluorescence were obtained from Abcam (Cambridge, UK). Low Glucose Dulbecco’s Modified Eagle’s Medium (DMEM), streptomycin double-antibody, and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY, USA). Paraformaldehyde and phosphate-buffered saline (PBS) were obtained from Coolabar (Beijing, China). Cell Counting Kit-8 (CCK-8) and Calcein-AM/propidium iodide (PI) were supplied by Beyotime Biotechnology (Shanghai, China). The osteogenic medium used for alizarin red dye was obtained from Cyagen (Santa Clara, CA, USA). The Perfect Real-Time RE reagent kit from Takara Bio (Dalian, China), 2× Fast SYBR Green Master Mix from Roche Diagnostics (Basel, Switzerland), and TRIzol reagent (Invitrogen, CA, USA) were used. The Runx-2, OPN, and RANKL antibodies were supplied by Abcam (Cambridge, UK) and DAPI from Solarbio (Beijing, China). The Electrochemical Immunoassay Kit was obtained from Roche-Mannheim (Mannheim, Germany). The tartrate-resistant acid phosphatase (TRAP) staining kit was obtained from Sigma-Aldrich (St. Louis, MO, USA). The ultrapure water used in the study was obtained from a Milli-QA10 filtration system (Millipore, Billerica, MA, USA).

The Ti6Al4V powder was obtained from AK Medical Co., Ltd. (Beijing, China). The low Glucose Dulbecco’s Modified Eagle’s Medium (DMEM), streptomycin–penicillin dual Antibiotics, and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, United States). Pareformaldehyde and Phosphate buffer (PBS) were obtained from Solarbio (Beijing, China). The Live-Dead staining kit was obtained from Bioss (Beijing, China). The Hematoxylin and Eosin (H&E) stain, Masson’s trichrome stain, and Van Gieson (VG) stain were purchased from Thermo Fisher Scientific (Shanghai, China). Rhodamine phalloidin and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Thermo Fisher Scientific (Shanghai, China). The double-distilled water used in this study was obtained from a Milli-QA10 filtration system (Milipore, Billerican, MA, United States). BMP-2 and OCN antibodies used in immunofluorescence were purchased from Abcam (Cambridge, United Kingdom).