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Keenview 2 digital camera

Manufactured by Olympus
Sourced in Germany, Japan

The KeenView II is a digital camera designed for laboratory use. It features a high-resolution sensor and advanced optics to capture clear, detailed images. The camera is compatible with a range of microscopes and other scientific instruments, making it a versatile tool for various imaging applications.

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2 protocols using keenview 2 digital camera

1

Quantifying Mitochondrial Morphology and Ultrastructure

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Samples were subjected to electron microscopy with minor variations from Zischka et al. (2008) (link). Briefly, 3×105 cells were immediately pelleted, fixed in 2.5% glutaraldehyde, fixed with 1% osmium tetroxide, dehydrated with acetone, and embedded in EPON resin. Ultra-thin sections were stained with lead citrate and UranyLess and imaged on a 1200EX electron microscope (JEOL, Tokyo, Japan) at 60 kV. Electron micrographs were recorded with a KeenView II digital camera (Olympus, Munich, Germany) with iTEM Ver. 5.0 (analySIS FIVE, Olympus, Munich, Germany).
Mitochondrial roundness was quantified as described in Tobias et al. (2018) (link) with ImageJ (V1.52i). Briefly, at least 150 mitochondria per condition were encircled with the polygonal section tool and fitted to an ellipse. Outliers were identified and removed with GraphPad Prism by the ROUT method at Q=1%. Mitochondrial cristae density was quantified by encircling 100 mitochondria per condition and manually counting the cristae, which were then divided by mitochondrial area. Lysosomes were counted manually in 100 µm2 rectangular sections, each composed from four 25 µm2 adjacent sections from the same slice. At least eight composite sections per condition were analyzed.
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2

Ultrastructural Analysis of Mitochondria

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Animal livers and isolated mitochondria were fixed with 2.5% glutaraldehyde (Science Services GmbH), postfixed with 1% osmium tetroxide, dehydrated with ethanol and propylene oxide, and were embedded in epoxy resin. Sixty-nanometer sections were cut using the Leica EM UC7 microtome (Leica Biosystems) or the Reichert-Jung Ultracut E microtome (now Leica Biosystems). Ultrathin sections were negative-stained with uranyl acetate (Uranyless) and lead citrate. Images were acquired using either a FEI Tecnai-12 electron microscope equipped with a VELETTA CCD digital camera (FEI, Eindhoven, The Netherlands) or using a Jeol 1200 EXII electron microscope (Akishima, Tokyo, Japan) equipped with a KeenViewII digital camera (Olympus, Hamburg, Germany) and processed with the iTEM software package (anlySISFive; Olympus).
For structural analyses, mitochondria were grouped in normally structured mitochondria of the “condensed type”59 (link) or in altered mitochondria with marked membrane detachments, matrix condensations, and ballooned cristae. A total of 350–750 mitochondria were included per group of animals.
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