Mitochondrial roundness was quantified as described in Tobias et al. (2018) (link) with ImageJ (V1.52i). Briefly, at least 150 mitochondria per condition were encircled with the polygonal section tool and fitted to an ellipse. Outliers were identified and removed with GraphPad Prism by the ROUT method at Q=1%. Mitochondrial cristae density was quantified by encircling 100 mitochondria per condition and manually counting the cristae, which were then divided by mitochondrial area. Lysosomes were counted manually in 100 µm2 rectangular sections, each composed from four 25 µm2 adjacent sections from the same slice. At least eight composite sections per condition were analyzed.
Keenview 2 digital camera
The KeenView II is a digital camera designed for laboratory use. It features a high-resolution sensor and advanced optics to capture clear, detailed images. The camera is compatible with a range of microscopes and other scientific instruments, making it a versatile tool for various imaging applications.
Lab products found in correlation
2 protocols using keenview 2 digital camera
Quantifying Mitochondrial Morphology and Ultrastructure
Mitochondrial roundness was quantified as described in Tobias et al. (2018) (link) with ImageJ (V1.52i). Briefly, at least 150 mitochondria per condition were encircled with the polygonal section tool and fitted to an ellipse. Outliers were identified and removed with GraphPad Prism by the ROUT method at Q=1%. Mitochondrial cristae density was quantified by encircling 100 mitochondria per condition and manually counting the cristae, which were then divided by mitochondrial area. Lysosomes were counted manually in 100 µm2 rectangular sections, each composed from four 25 µm2 adjacent sections from the same slice. At least eight composite sections per condition were analyzed.
Ultrastructural Analysis of Mitochondria
For structural analyses, mitochondria were grouped in normally structured mitochondria of the “condensed type”59 (link) or in altered mitochondria with marked membrane detachments, matrix condensations, and ballooned cristae. A total of 350–750 mitochondria were included per group of animals.
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