Augmentin

After 3 days of co-cultivation, ‘Micro-Tom’ cotyledon segments were placed on callus-induction medium (MS medium containing 0.3% Gelrite, 1.5 mg l⁻¹ zeatin, 100 mg l⁻¹ kanamycin, and 375 mg l⁻¹ augmentin [GlaxoSmithKline, MDX, UK]) for 4 weeks. Calli that formed from the segments were cultured on shoot-induction medium (MS medium containing 0.3% Gelrite, 1.0 mg l⁻¹ zeatin, 100 mg l⁻¹ kanamycin, and 375 mg l⁻¹ augmentin) for 4 weeks. The shoots were then placed on rooting medium, which consisted of half-strength MS medium, 0.3% Gelrite (Wako, Tokyo, Japan), 100 mg l⁻¹ kanamycin, and 375 mg l⁻¹ augmentin, for 2 weeks. Tissues were subcultured every 10–14 days. The ploidy of the rooting shoots was checked via flow cytometry.

Tomato transformations followed the protocol by Sun et al. (2006) (link). In brief, after 3 days of co-cultivation, tomato cotyledon segments were placed on a callus-induction medium [MS medium containing 0.3% Gelrite (Wako, Tokyo, Japan), 1.5 mg/l zeatin, 100 mg/l kanamycin, and 375 mg/l Augmentin (GlaxoSmithKline, London, UK)] for 4 weeks. Calli that formed segments were cultured on shoot-induction medium [MS medium containing 0.3% Gelrite (Wako, Tokyo, Japan), 1.0 mg/l zeatin, 100 mg/l kanamycin, and 375 mg/l Augmentin (GlaxoSmithKline, London, UK)] for 4 weeks. The shoots were then placed on rooting medium, which consisted of half-strength MS medium, 0.3% Gelrite (Wako, Tokyo, Japan), 100 mg/L kanamycin, and 375 mg/l Augmentin, for 2 weeks. Tissues were each subcultured for 10–14 days.