Alexa fluor 488 labeled goat anti mouse a0428

Primary antibodies against the following proteins were used in this study: PKR (BS3653), phospho-PKR^T446 (BS4789), and myxovirus resistance (MX)1 (BS6674) (all from BioWorld, Visalia, CA, USA); eIF2α (SC-11386) and phospho-eIF2α^S51 (SC-12412) (both from Santa Cruz Biotechnology, Santa Cruz, CA, USA); CSFV E2 (WH303; Median Diagnostics, Chuncheon, Korea); β-actin (Beyotime, Beijing, China, AA128); and green fluorescent protein (GFP) (1533-1; Epitomics, Burlingame, CA, USA). Horseradish peroxidase-conjugated goat anti-rabbit (BS13278) and anti-mouse (BS12478) secondary antibodies were from BioWorld. Alexa Fluor 555-labeled donkey anti-rabbit (A0453) and Alexa Fluor 488-labeled goat anti-mouse (A0428) secondary antibodies were from Beyotime. 2-Aminopurine (2-AP; A2380) and poly(I:C) (P1530) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The pEGFP-PKR and pEGFP-N1 vectors were prepared in our laboratory (Tian and Mathews, 2001 (link)). Monoclonal antibodies against the NS5A, NS3, and N^pro proteins of CSFV were provided by Dr. Xinglong Yu (Veterinary Department, Hunan Agricultural University, China). PKR and scrambled short hairpin (sh)RNA were designed by Cyagen Biosciences Inc. (Santa Clara, CA, USA). The PKR shRNA sequence 5′-GCA GAA CTT CTT CAC ATA TGT-3′ was inserted into the PLenti X1-puro-shRNA-eGFP vector.