Transmission electron microscopy (TEM) was used for the determination of the phage morphology. A filtered high-titer (about 1011 PFU/mL) phage stock solution was deposited on a Formvar- and carbon-coated grid (Agar Scientific, Essex, UK) and incubated for 30 s. The excess liquid was removed with a filter paper before the grid was negative stained with phosphor tungstic acid (5%) for 30 s. Phages were examined using a JEM-2100F TEM (JEOL, Tokyo, Japan) in SIB Labs, University of Eastern Finland, Finland.
Formvar and carbon coated grid
Manufactured by Agar Scientific
Sourced in United Kingdom
Formvar- and carbon-coated grid is a type of laboratory equipment used to support and prepare samples for electron microscopy analysis. It provides a thin, uniform support film that helps to maintain the structural integrity of the sample during the imaging process.
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Lab products found in correlation
2 protocols using formvar and carbon coated grid
1
Phage Morphology Analysis by TEM
2
Fibril Characterization by TEM
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Lyophilized powder was dissolved in 0.1 M phosphate buffer (pH 5) to a final concentration of 1 mg/mL. Prepared sample was filtered through 0.2 mm filter (Iso-Disc TM ) and incubated at 50 °C and 300 rpm for 96 h. Undiluted sample was applied to Formvar and carbon-coated grid (Agar Scientific, Stansted, UK) and left to adsorb for 3 min. The excess of sample was soaked away and stained with 1% (w/v) water solution of uranyl acetate. The excess of stain was removed immediately. The fibrils were observed with a Philips (Amsterdam, the Netherlands) CM 100 transmission electron microscope at 80 kV. Images were recorded by Gatan Orius SC200 CCD camera and Digital Micrograph software 3.1, (Gatan Inc., Washington, DC, USA).
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