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Hsp22 sirna s206904 1 and s96094 2

Manufactured by Thermo Fisher Scientific
Sourced in United States

HSP22-siRNA (s206904 (#1) and s96094 (#2)) are two different siRNA (small interfering RNA) sequences targeting the HSP22 (Heat Shock Protein 22) gene. siRNAs are used to silence or knockdown the expression of specific genes in cells. No further details on the intended use or application of these particular siRNA products are provided.

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3 protocols using hsp22 sirna s206904 1 and s96094 2

1

PGF2α Signaling Pathway Regulation

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PGF2α was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The ELISA kits for mouse IL-6 and mouse VEGF were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Phospho-specific p44/p42 MAP kinase antibodies, p44/p42 MAP kinase antibodies, phospho-specific p38 MAP kinase antibodies, and p38 MAP kinase antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Buckinghamshire, UK). Negative control-small interfering RNA (siRNA) (Silencer Negative Control #1 siRNA (Neg)) and HSP22-siRNA (s206904 (#1) and s96094 (#2)) were purchased from Ambion (Austin, TX, USA). Other materials and chemicals were obtained from commercial sources.
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2

Investigating Heat Shock Protein 22 Function

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PGF2a was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The ELISA kits for mouse IL-6 and mouse VEGF were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Phospho-specific p44/p42 MAP kinase antibodies, p44/p42 MAP kinase antibodies, phospho-specific p38 MAP kinase antibodies and p38 MAP kinase antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Glyceraldehyde-3phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Buckinghamshire, UK). Negative control-small interfering RNA (siRNA) (Silencer Negative Control #1 siRNA (Neg)) and HSP22-siRNA (s206904 (#1) and s96094 (#2)) were purchased from Ambion (Austin, TX, USA).
Other materials and chemicals were obtained from commercial sources.
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3

PGF2-Induced MAPK Signaling Pathway

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PGF2 was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). The ELISA kits for mouse IL-6 and mouse VEGF were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Phospho-speci c p44/p42 MAP kinase antibodies, p44/p42 MAP kinase antibodies, phospho-speci c p38 MAP kinase antibodies and p38 MAP kinase antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Buckinghamshire, UK). Negative control-small interfering RNA (siRNA) (Silencer Negative Control #1 siRNA (Neg)) and HSP22-siRNA (s206904 (#1) and s96094 (#2)) were purchased from Ambion (Austin, TX, USA). Other materials and chemicals were obtained from commercial sources.
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