The bioreactor was sampled daily for off-line measurements. Total cell density, cell viability, and cell size were measured using a cell counter (Vi-cell Beckman Coulter, Fullerton, CA). Glucose, lactate, ammonium, glutamine, and other metabolites were measured with a NOVA Flex BioProfile analyzer (NOVA biomedical). pH was measured using Seven Excellence Multiparameter (METTLER TOLEDO). Oxygen (pO2) and carbon dioxide (pCO2) partial pressure were measured using a Siemens Blood Gas Analyzer RapidLab 248. Technical replicate samples of 15 mL were collected daily from pre-inoculation day to day 13, centrifuged at 2000 r.c.f. for 10 min, filtered through a 0.2 µm polyethersulfone filter (Pall), and stored at −80 °C in 500 µL aliquots in matrix tubes (Thermo Fisher) for further analysis. Technical replicate bulk sampling of 100 mL aliquots was performed from day 5 to day 13. On termination day (day 13), 2 L of the total working volume was collected and centrifuged at 2000 r.c.f. for 60 min, filtered through a 0.2 µm polyethersulfone filter (Pall), and stored at −80 °C.
Blood gas analyzer rapidlab 248
Manufactured by Siemens
The Siemens RapidLab 248 is a blood gas analyzer that measures the levels of oxygen, carbon dioxide, and other related parameters in blood samples. It is designed to provide rapid and accurate analysis of blood gas levels.
Automatically generated - may contain errors
2 protocols using blood gas analyzer rapidlab 248
1
Bioreactor Cell Culture Sampling
2
Bioreactor Cell Culture Monitoring
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The bioreactor was sampled daily for off-line measurements. Total cell density, cell viability, and cell size were measured using a cell counter (Vi-cell Beckman Coulter, Fullerton, CA). Glucose, lactate, ammonium, glutamine, and other metabolites were measured with a NOVA Flex BioProfile analyzer (NOVA biomedical). pH was measured using Seven Excellence Multiparameter (METTLER TOLEDO). Oxygen (pO2) and carbon dioxide (pCO2) partial pressure were measured using a Siemens Blood Gas Analyzer RapidLab 248. Technical replicate samples of 15 mL were collected daily from pre-inoculation day to day 13, centrifuged at 2000 rcf for 10 min, filtered through a 0.2 µm polyethersulfone filter (Pall), and stored at -80 °C in 500 µL aliquots in matrix tubes (Thermo Fisher) for further analysis. Technical replicate bulk sampling of 100 mL aliquots was performed from day 5 to day 13. On termination day (day 13), 2 L of the total working volume was collected and centrifuged at 2000 rcf for 60 min, filtered through a 0.2 µm polyethersulfone filter (Pall), and stored at -80 °C.
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