Whatman puradisctm25as polyethersulfone membrane

To examine the inhibitory effect of aqueous leachates of the six Chinese chive cultivars on FOC colony growth, the fresh aerial parts (50 g) were also ground into powder in liquid nitrogen and soaked in 100 mL distilled water at 25 °C for 20 min for extractions by an ultrasonic agitation, respectively ( Zuo et al 2016) . Then aqueous solution was centrifuged at 10000 rpm for 5 min. After centrifugation, the supernatant was ltered rst through normal lter paper (0.45 µm, Xingya Puri cation Factory, Shanghai, China) and then through a micro lter of pore size 0.22 µm (Whatman puradiscTM25AS polyethersulfone membrane, catalog NO.6794 - 2514, England). The nal ltrate was our original leachates with a concentration of 0.5 g FW•mL - 1 .
The ltered leachates (5 mL) of each cultivar and fungal culture medium (5 mL) were mixed and added in petri dishes (90 mm in diameter) and inoculated with a 4 mm discs of FOC. The concentration of nal culture medium was 0.25 g FW•mL - 1 . Similar petri dish without Chinese chive leachate but with the same volume of distilled water were used as controls. Six replicates were prepared for each treatment.
The experiment was conducted in a climate-controlled room at 25 °C in darkness. Colony diameters of the inoculated FOC were measured with a Vernier caliper when the colony of the control had reached the peripheral wall.

To examine the inhibitory effect of aqueous leachates of the six Chinese chive cultivars on FOC colony growth, the fresh aerial parts (50 g) were also ground into powder in liquid nitrogen and soaked in 100 mL distilled water at 25°C for 20 min for extractions by an ultrasonic agitation, respectively ( Zuo et al 2016) . Then, the aqueous solution was centrifuged at 10000 rpm for 5 min. After centrifugation, the supernatant was ltered rst through normal lter paper (0.45 μm, Xingya Puri cation Factory, Shanghai, China) and then through a micro lter of pore size 0.22 μm (Whatman puradiscTM25AS polyethersulfone membrane, catalog NO.6794-2514, England). The nal ltrate was our original leachates with a concentration of 0.5 g FW•mL -1 .
The ltered leachates (5 mL) of each cultivar and fungal culture medium (5 mL) were mixed and added in petri dishes (90 mm in diameter) and inoculated with a 4 mm discs of FOC. The concentration of nal culture medium was 0.25 g FW•mL -1 . Similar petri dish without Chinese chive leachate but with the same volume of distilled water was used as controls. Six replicates were prepared for each treatment.
The experiment was conducted as described in the above methods part 'Inhibitory effect of leaf volatiles'.