Immunohistochemistry assay was performed as described by Xu. et al. [18 (link)]. In brief, serial cross sections of 4 μm thick were collected and stained immunohistochemically according to the manufacturer's instructions. The samples were deparaffinized, rehydrated, and incubated with fresh 0.3% hydrogen peroxide in methanol for 10 min at 37°C. The sections were then autoclaved for antigen retrieval in citrate buffer at 100°C for 5 min and incubated with indicated antibodies overnight at 4°C. The sections were washed with phosphate-buffered saline (PBS) and incubated with biotinylated anti-rabbit IgG as a secondary antibody for 15 min at 37°C and then with streptavidin-conjugated horseradish peroxidase for 15 min at 37°C (Zymed, Carlsbad, USA). The immune reaction was demonstrated using DAB, and the sections were counterstained with hematoxylin, dehydrated, and then mounted.
Streptavidin conjugated horseradish peroxidase
Manufactured by Zymo Research
Sourced in United States
Streptavidin-conjugated horseradish peroxidase is a detection reagent that combines the high-affinity binding of streptavidin to biotin with the enzymatic activity of horseradish peroxidase. This conjugate is commonly used in various immunoassay and detection techniques.
Automatically generated - may contain errors
Lab products found in correlation
Ab41902, by Abcam (1 mentions)
Histostain plus kit, by Zymo Research (1 mentions)
Goat serum, by Thermo Fisher Scientific (1 mentions)
Biotinylated anti rabbit igg antibody, by Vector Laboratories (1 mentions)
Fast red, by Merck Group (1 mentions)
Diaminobenzidine dab, by Merck Group (1 mentions)
Biotinylated pna, by Vector Laboratories (1 mentions)
4 protocols using streptavidin conjugated horseradish peroxidase
1
Immunohistochemical Staining Protocol
2
Immunohistochemical Analysis of LXRα in Oral Cancer
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Serial cross sections, 4 μm thick, were collected and stained immunohistochemically as per manufacturer's instructions for the Histostain®-Plus kits (Zymed, Carlsbad, USA). The samples were deparaffinized, rehydrated, and incubated with fresh 0.3% hydrogen peroxide in methanol for 10 min at 37 °C. Sections were autoclaved for antigen retrieval in citrate buffer at 100 °C for 2 min and incubated with rabbit LXRα polyclonal antibody (ab41902, dilution 1:1000, Abcam, USA) at 4 °C overnight. Sections were washed with PBS and incubated with biotinylated anti-rabbit IgG as a second antibody for 15 min at 37 °C and then with streptavidin-conjugated horseradish peroxidase for 15 min at 37 °C (Zymed, Carlsbad, USA). An immune reaction was demonstrated with DAB. The sections were counterstained with hematoxylin, dehydrated, and mounted. PBS substituted for primary antibody in negative controls, with no evident detectable staining. LXRα -positive oral cancer was a positive control in this study.
3
Immunohistochemical Localization of UBE3B
4
Spleen Analysis of Sca1-Bcl6 Mice
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