Dipalmitoyl sn glycero 3 phosphocholine

Manufactured by Avanti Polar Lipids

Sourced in United States

Dipalmitoyl-sn-glycero-3-phosphocholine is a synthetic phospholipid. It is a major component of biological membranes and is commonly used in liposome and lipid bilayer research.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

6 protocols using dipalmitoyl sn glycero 3 phosphocholine

Lipid Composition for Nanoparticle Formulations

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-ditetradecanoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (PEG2000-DSPE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)2000] (Biotin-PEG2000-DSPE), 1,2-Dioleoyl-sn-Glycero-3-Phosphoethanolamine-N-(Lissamine Rhodamine B Sulfonyl) (Rhodamine PE), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (PEG2000-DSPEmal), 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) and cholesterol were purchased from Avanti Polar Lipids (Alabaster, AL). Gd(III)DOTA-DSPE and Gd(III)-DTPA-bis(stearylamide) (Gd(III)-DTPA-BSA) were obtained from Gateway Chemical Technology (St. Louis, MO).

Kuijten M.M., Hannah Degeling M., Chen J.W., Wojtkiewicz G., Waterman P., Weissleder R., Azzi J., Nicolay K, & Tannous B.A. (2015). Multimodal targeted high relaxivity thermosensitive liposome for in vivo imaging. Scientific Reports, 5, 17220.

+ Open protocol

+ Expand

Phospholipid-Brominated Flame Retardant Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

All phospholipids used in the studies are
the following: 1,2-dioleoyl-sn-glycero-3-phosphocholine
(DOPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
(DOPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
(POPE), 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine
(DPPE), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol)
sodium salt (DOPG), and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol)
sodium salt (DPPG) were purchased from Avanti Polar Lipids. All the
phospholipids were powdered, lyophilized samples, of 99% purity. BFRs
used in the studies: 3,3′,5,5′-tetrabromobisphenol A
(TBBPA), 2,2′,4,4′,5-pentabromodiphenyl ether (BDE 99),
1,2,5,6,9,10-hexabromocyclododecane (HBCD), 1,2,5,6-tetrabromocyclooctane
(TBCO), 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE), 2,4,6-tribromophenol
(TBP), and 2,3,4,5,6-pentabromotoluene (PBT) were purchased from Merck
Signa-Aldrich. All the BFRs were analytical standards of purity >99%.
The applied organic solvents: chloroform (99.5%, HPLC grade) and methanol
(99.5%, HPLC grade) were purchased from Merck Sigma-Aldrich. Ultrapure
water of 18.2 MΩ·cm resistivity was produced in our laboratory
with the application of a Merck MilliPore water purification system.

Broniatowski M, & Wydro P. (2024). Interactions of Brominated Flame Retardants with Membrane Models of Dehalogenating Bacteria: Langmuir Monolayer and Grazing Incidence X-ray Diffraction Studies. Langmuir, 40(20), 10600-10614.

+ Open protocol

+ Expand

Phospholipid Characterization for Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,2-dilinoleoyl-sn-glycero-3-phosphocholine (14:1),
1,2-dipalmitoleoyl-sn-glycero-3-phosphocholine (16:1),
1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC/18:1 cis),
1,2-dielaidoyl-sn-glycero-3-phosphocholine (18:1 trans),
1,2-dieicosenoyl-sn-glycero-3-phosphocholine (20:1),
1,2-dierucoyl-sn-glycero-3-phosphocholine (22:1),
1,2-dinervonoyl-sn-glycero-3-phosphocholine (24:1),
1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC/14:0),
1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC/16:0),
1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC/18:0),
1,2-dilinoleoyl-sn-glycero-3-phosphocholine (18:2), and
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC/16:0–18:1 cis)
were purchased from Avanti Polar Lipids (Alabaster, AL). All other reagents were
ordered from Sigma-Aldrich (St. Louis, MO). RPMI-1640 cell culture media was
purchased from ATCC (Manassas, VA). Fetal bovine serum, Penicillin/Streptomycin,
and Alexa Fluor 488 NHS Ester (AF488) were obtained from Invitrogen (Carlsbad,
CA).

Gilmore S.F., Carpenter T.S., Ingólfsson H.I., Peters S.K., Henderson P.T., Blanchette C.D, & Fischer N.O. (2018). Lipid composition dictates serum stability of reconstituted high-density lipoproteins: implications for in vivo applications. Nanoscale, 10(16), 7420-7430.

+ Open protocol

+ Expand

Lipid-based Nanoparticle Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The lipids dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG2000) and cholesterol were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). DSPE-PEOz2000 was purchased from Ruixi Biological Technology (Xi’an, Shanxi, China). IR780 iodide and metformin were purchased from Aladdin (Shanghai, China) and Sigma-Aldrich (St. Louis, Missouri, USA), respectively. Cell Counting Kit-8 (CCK-8) was purchased from Meilunbio^® (Dalian, Liaoning, China). 2-(4-Amidino-phenyl)-6-indolecarbamidinedihydrochloride (DAPI), 1,3-diphenylisobenzofuran (DPBF) and 2ʹ,7ʹ-dichlorodihydrofluorescein diacetate (DCFH-DA) were obtained from Beyotime Technology (Shanghai, China). Deionized water was obtained from Millipore water purification systems. All reagents and solvents used in this subject were purchased commercially and used without further purification unless specifically stated in the instructions.

Zhang N., Tan Y., Yan L., Zhang C., Xu M., Guo H., Zhuang B., Zhou L, & Xie X. (2020). Modulation of Tumor Hypoxia by pH-Responsive Liposomes to Inhibit Mitochondrial Respiration for Enhancing Sonodynamic Therapy. International Journal of Nanomedicine, 15, 5687-5700.

+ Open protocol

+ Expand

Lipid Nanoparticle Formulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals unless otherwise specified were obtained from Sigma–Aldrich. The lipid dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was purchased from Avanti Polar Lipids. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine N-[methoxy(polyethylene glycol)-5000] (DSPE-PEG) was purchased from Laysan Bio Inc. Human recombinant insulin (Zn salt, 27.5 IU/mg) was obtained from Life Technology.

Zhu Y.S., Yen P.M., Chin W.W, & Pfaff D.W. (1996). Estrogen and thyroid hormone interaction on regulation of gene expression.. Proceedings of the National Academy of Sciences of the United States of America, 93(22), 12587-12592.

+ Open protocol

+ Expand

Intranasal Liposomal Vaccine Formulation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Liposomal vaccines were prepared as per established methods^{11 (link),13 (link)}. Predetermined amounts of J8-palmitic acid, 3D(6-acyl) PHAD (PHAD; Avanti Polar Lipids Inc., USA) and phospholipids—dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol (CHOL) and 1 L-α-phosphatidylglycerol (PG) (Avanti Polar Lipids Inc.) were coated on to round bottom flasks to form a lipid thin film. The thin films were hydrated in 20 mM phosphate-buffered saline (PBS; pH 6) containing trehalose and Diphtheria Toxoid (DT). The resultant liposomal suspension was freeze-dried and vaccines were stored at 4 °C until use. The average particle size (nm) of a liposome was measured using a Nanosizer (Zetasizer Nano Series ZS, Malvern Instruments) as per established method^{11 (link)}. Homogenous size distribution was confirmed by a low polydispersity index (PDI) of 0.08 for J8-Lipo-DT-PHAD with an average size of 1987 nm (SD 104 nm) before freeze-drying and PDI of 0.16 with an average size of 5213 nm (SD 572.2) after reconstitution of freeze-dried liposomes in PBS. For administration, each vaccine vial was resuspended in PBS, and mice were immunized intranasally (i.n.) with a total volume of 20 μl (30 μg/mouse of J8-palmitic acid, 25 μg/mouse of PHAD and 30 μg/mouse of DT—10 μl/nare).

Ozberk V., Zaman M., Lepletier A., Eskandari S., Kaden J., Mills J.L., Calcutt A., Dooley J., Huo Y., Langshaw E.L., Ulett G.C., Batzloff M.R., Good M.F, & Pandey M. (2023). A Glycolipidated-liposomal peptide vaccine confers long-term mucosal protection against Streptococcus pyogenes via IL-17, macrophages and neutrophils. Nature Communications, 14, 5963.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!