Rin 5f

Human pancreatic ductal adenocarcinoma cell lines PANC-1, MIA PaCa-2, and RIN-5F were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA), and BxPC-3 was purchased from Perkin Elmer (Waltham, MA, USA). PANC-1 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific Inc., Waltham, MA, USA) and 1% penicillin-streptomycin-amphotericin B solution (PSA) (BioVision Inc., Milpitas, CA, USA). MIA PaCa-2 cells were cultured in DMEM medium supplemented with 10% FBS (Gibco; Thermo Fisher Scientific Inc., Waltham, MA, USA), 2.5% horse serum (HS) (Gibco; Thermo Fisher Scientific Inc., Waltham, MA, USA), and 1% PSA (BioVision Inc., Milpitas, CA, USA). RIN-5F cells were culture in RPMI-1640 medium supplemented with 10% FBS and 1% PSA. BxPC-3 cells were maintained in 1640-RPMI medium containing 10% FBS (Gibco; Thermo Fisher Scientific Inc., Waltham, MA, USA) and 1% PSA (BioVision Inc., Milpitas, CA, USA). Cells were cultured in an atmosphere of 95% air and 5% CO₂ at 37 °C [33 (link),34 (link)].

The pancreatic beta cell RIN-5F was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). RIN-5F cells were cultured in RPMI-1640 medium (Wako, Tokyo, Japan) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 100 U/ml of penicillin, and 100 µg/ml of streptomycin (Wako). Cells were cultured at 37 °C in a humidified atmosphere with 5% CO₂.

Rat pancreatic beta cell line (RIN-5F) and rat L6 myoblast cell line (L6) were used in this study. RIN-5F and L6 cells were purchased from the American Type Culture Collection (ATCC, USA). RIN-5F (ATCC, CRL- 2058) cell were cultured in RPMI-1640 (Sigma–Aldrich, St. Louis, MO, USA) and L6 cells (ATCC, CRL-1458) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies, Inc., Rockville, MD, USA). The cells were supplemented with 10% fetal bovine serum (FBS, Sigma–Aldrich, St. Louis, MO, USA) and 1% antibiotics (100 IU/mL of penicillin and 100 μg/mL of streptomycin (iDNA, South America) and were maintained in a humidified 5% CO2 incubator at 37 °C. Cells were seeded in a flask at the required density per well and incubated for the desired time prior to the experiments.