Prnat u6 neo vector

Manufactured by GenScript

Sourced in United States

The PRNAT-U6/Neo vector is a plasmid designed for gene expression and gene silencing studies. It contains a U6 promoter for driving shRNA expression and a neomycin resistance gene for selection in mammalian cells.

Automatically generated - may contain errors

Lab products found in correlation

Human dermal fibroblast nucleofector kit, by Lonza (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

2 protocols using prnat u6 neo vector

Transfection of CDKN2A, p16, and p53 in fibroblasts

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CDKN2A-shRNA expressed in pRNAT-U6/Neo vector (GenScript Corporation) and the corresponding control plasmid were used at 1 μg/mL for transfection using human dermal fibroblast nucleofector kit (Amaxa Biosystems) following the protocol recommended by the manufacturer.
pIRES-Puro2 encoding FLAG-tagged human p16 wild-type or PLVX-Puro-Flag-tagged mutants,^{31 (link)} pC53SN3 encoding wild-type human p53^{32 (link)} or pCMVHup53Cdelta30 encoding mutant p53 (CΔ30)^{33 (link)} and their control plasmids were used at 1 μg/mL for transfection using Lipofectamine 2 000 following the manufacturer’s instructions (Invitrogen, Carlsbad, CA).

Al-Khalaf H.H., Nallar S.C., Kalvakolanu D.V, & Aboussekhra A. (2017). p16INK4A enhances the transcriptional and the apoptotic functions of p53 through DNA-dependent interaction. Molecular carcinogenesis, 56(7), 1687-1702.

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Knockdown and Overexpression of miR-146b-5p

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CDKN2A-shRNA expressed in pRNAT-U6/Neo vector (GenScript Corporation) and the corresponding control plasmid were used to carry out transfection using human dermal fibroblast nucleofector kit (Amaxa Biosystems) following the protocol recommended by the manufacturer.
pLKO.1-miRZip146b-5p and pCDH-pre-miR-146b-5p plasmids were purchased from System Biosciences (SBI, USA) and used to prepare the lentiviral supernatant. Media was removed from the target cells and replaced with lentiviral supernatant and incubated for 24 h.

Al-Ansari M.M, & Aboussekhra A. (2015). miR-146b-5p mediates p16-dependent repression of IL-6 and suppresses paracrine procarcinogenic effects of breast stromal fibroblasts. Oncotarget, 6(30), 30006-30016.

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