HCC2429 (Dr. Thao P. Dang), Ty-82 (JCRB Cell Bank), 10–15 (15 ), and 14169 cells were maintained in RPMI 1640 (Invitrogen) with 10% fetal bovine serum (Hyclone) and 1% penicillin/streptomycin (Invitrogen). The 10–15 and 14169 cells were provided by Christopher French. All four cell lines have been authenticated using BRD4-NUT western blotting, Immunofluorescent staining, and DNA sequencing as described in the manuscript.
The non-target control, BRD4, NUT, and SOX2 siRNAs were purchased from Dharmacon. For siRNA knockdown, cells were transfected using DharmaFECT reagents (Thermo Scientific). For SOX2 double knockdown, cells were re-seeded at 24 h after the first siRNA transfection and re-transfected with the same siRNA 12 h later. HCC2429 SOX2 stable cells were generated as described inSupplementary Methods .
The non-target control, BRD4, NUT, and SOX2 siRNAs were purchased from Dharmacon. For siRNA knockdown, cells were transfected using DharmaFECT reagents (Thermo Scientific). For SOX2 double knockdown, cells were re-seeded at 24 h after the first siRNA transfection and re-transfected with the same siRNA 12 h later. HCC2429 SOX2 stable cells were generated as described in