200 proof ethanol

Manufactured by Thermo Fisher Scientific

Sourced in United States

200-proof ethanol is a high-purity, anhydrous form of ethyl alcohol. It is a clear, colorless liquid with a characteristic odor. 200-proof ethanol has a purity level of 100% and is commonly used as a solvent, reagent, and in various laboratory applications that require an extremely pure alcohol solution.

Automatically generated - may contain errors

Lab products found in correlation

16 protocols using 200 proof ethanol

Cleaning and Preparing Glass Slides for Thin-Film Deposition

Check if the same lab product or an alternative is used in the 5 most similar protocols

A No. 1.5H ibidi glass coverslip (25 × 75 mm) (ibidi #10812) was cleaned by sonication in DI water for five minutes. The sample was then subjected to a second sonication in fresh DI water for five minutes. Finally, the slide was sonicated in 200 proof ethanol (Fischer Scientific #04-355-223) for five minutes and was subsequently dried under a stream of N₂. The cleaned glass coverslip was then mounted into a home-built thermal evaporator chamber in which the pressure was reduced to 50 × 10⁻³ Torr. The chamber was purged with N₂ three times and the pressure was reduced to 1–2 × 10⁻⁷ Torr by using a turbo pump with a liquid N₂ trap. Once the desired pressure was achieved, a 3 nm film of Cr was deposited onto the slide at a rate of 0.2 Å s⁻¹, which was determined by a quartz-crystal microbalance. After the Cr adhesive layer had been deposited, 6 nm of Au was deposited at a rate of 0.4 Å s⁻¹. The Au-coated samples were used within one week of deposition.

Piranej S., Bazrafshan A, & Salaita K. (2022). Chemical-to-mechanical molecular computation using DNA-based motors with onboard logic. Nature nanotechnology, 17(5), 514-523.

+ Open protocol

+ Expand

Electrochemical Biosensor for SARS-CoV-2 Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Graphite flakes, ethyl cellulose (4x10⁻³ Pa/s), terpineol, N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS), ethanolamine, 2-(N-morpholino) ethanesulfonic acid (MES buffer), potassium hexacyanoferrate (II) trihydrate, and potassium ferricyanide, were purchased from MilliporeSigma (Saint Louis, MO, USA). Potassium chloride was purchased from Fisher Scientific (Hampton, NH, USA). Superblock™ buffer and 200-proof ethanol were purchased from Thermo Fisher (Waltham, MA, USA). Artificial saliva was purchased from Pickering Laboratories (Mountain View, CA, USA). Phosphate buffer saline was purchased from Alfa Aesar (Tewksbury, MA, USA). SARS-CoV-2 Spike Rabbit polyclonal antibody (Cat. # 40591-T62), SARS-CoV-2 Spike RBD protein (Cat. # 40592-V08H), SARS-CoV-2 Spike S1 protein (Cat. # 40591-V08H), Middle East respiratory syndrome (MERS) Spike S1 protein (Cat. # 40069-V08H), and Influenza H1N1 hemagglutinin (HA) protein (Cat. #40006-V08H) were purchased from Sino Biological (Sino Biological US Inc., Wayne, PA, USA).

Pola C.C., Rangnekar S.V., Sheets R., Szydlowska B.M., Downing J.R., Parate K.W., Wallace S.G., Tsai D., Hersam M.C., Gomes C.L, & Claussen J.C. (2022). Aerosol-jet-printed graphene electrochemical immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva. 2d materials, 9(3), 035016.

+ Open protocol

+ Expand

Cell Culture and Molecular Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols

DMEM media, RPMI media, trypsin, fetal bovine serum (FBS), penicillin/streptomycin (P/S), L-glutamine, TRIzol, MTT reagent, and green SYBR reagent were obtained from Invitrogen (Carlsbad, CA, USA). Recombinant mouse interferon-γ (IFNγ) was obtained from R&D Systems (Minneapolis, MN, USA). Chloroform, isopropanol, DEPC water, and 200 proof ethanol were obtained from ThermoFisher (Waltham, MA, USA). Normal saline and phosphate buffered saline (PBS) were obtained from Corning Inc. (Corning, NY, USA). Sucrose and formalin solution were obtained from Sigma Aldrich (St. Louis, MO, USA).

Liaw K., Sharma R., Sharma A., Salazar S., La Rosa S.A, & Kannan R.M. (2020). Systemic Dendrimer Delivery of Triptolide to Tumor-Associated Macrophages Improves Anti-Tumor Efficacy and Reduces Systemic Toxicity in Glioblastoma. Journal of controlled release : official journal of the Controlled Release Society, 329, 434-444.

+ Open protocol

+ Expand

Glass Coverslip Preparation for Metal Deposition

Check if the same lab product or an alternative is used in the 5 most similar protocols

A No. 1.5H ibidi glass coverslip (25 × 75 mm) (ibidi #10812) was cleaned by sonication in DI water for five minutes. The sample was then subjected to a second sonication in fresh DI water for five minutes. Finally, the slide was sonicated in 200 proof ethanol (Fischer Scientific #04-355-223) for five minutes and was subsequently dried under a stream of N₂. The cleaned glass coverslip was then mounted into a home-built thermal evaporator chamber in which the pressure was reduced to 50 × 10⁻³ Torr. The chamber was purged with N₂ three times, and the pressure was reduced to 1–2 × 10⁻⁷ Torr by using a turbo pump with a liquid N₂ trap. Once the desired pressure was achieved, a 3 nm film of Cr was deposited onto the slide at a rate of 0.2 Å s⁻¹, which was determined by a quartz-crystal microbalance. After the Cr adhesive layer had been deposited, 6 nm of Au was deposited at a rate of 0.4 Å s⁻¹. The Au-coated samples were used within one week of deposition.

Piranej S., Zhang L., Bazrafshan A., Marin M., Melikyan G.B, & Salaita K. (2023). Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor. bioRxiv.

+ Open protocol

+ Expand

Mass Spectrometry Sample Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acetonitrile, α-Cyano-4-hydroxycinnamic acid (CHCA), trifluoroacetic acid (TFA), ammonium bicarbonate, ammonium phosphate monobasic, calcium chloride, and Trizma® base were purchased from Sigma-Aldrich (St. Louis, MO, USA). Collagenase type III (COLase3) (C. histolyticum) was purchased from Worthington (Lakewood, NJ, USA). Xylenes, 200 proof ethanol, methanol, HPLC grade water were purchased from Fisher Scientific (Pittsburgh, PA, USA).

Angel P.M., Bruner E., Bethard J., Clift C.L., Ball L., Drake R.R, & Feghali-Bostwick C. (2020). Extracellular Matrix Alterations in Low Grade Lung Adenocarcinoma Compared to Normal Lung Tissue by Imaging Mass Spectrometry. Journal of mass spectrometry : JMS, 55(4), e4450.

+ Open protocol

+ Expand

Tissue Dissociation and Extraction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Acetonitrile, α-Cyano-4-hydroxycinnamic acid (CHCA), trifluoracetic acid (TFA), sodium citrate, and trizma base were purchased from Sigma-Aldrich (St. Louis, MO, USA). Collagenase type III (COLase3) (C. histolyticum) and IV and elastase (MMP12; porcine) were purchased from Worthington Biochemicals (Lakewood, NJ), StemCell Technologies (Cambridge, MA), Thermo Scientific (Waltham, MA), Alfa Aesar (MMP12, Fisher Scientific, Pittsburgh, PA) or Abnova (Taipei City, Taiwan). Xylenes, 200 proof ethanol, methanol, citraconic anhydride were purchased from Fisher Scientific (Pittsburgh, PA).

Angel P.M., Comte-Walters S., Ball L.E., Talbot K., Mehta A., Brockbank K.G, & Drake R.R. (2017). Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-embedded Tissues by MALDI Imaging Mass Spectrometry. Journal of proteome research, 17(1), 635-646.

+ Open protocol

+ Expand

Synthesis of Osmium Complexes

Check if the same lab product or an alternative is used in the 5 most similar protocols

OsCl₃·3H₂O (99.95%) was purchased from Strem Chemicals. Dimethylformamide (DMF; 99.9%) and 200- proof ethanol was obtained from Fisher Scientific. ¹H NMR solvents CDCl₃, D₂O and Acetone-d₆ (99.9%) were obtained from Cambridge Isotope Lab Inc. The following chemicals were obtained from Sigma Aldrich: (NH₄)₂OsCl₆ (99.99%), 2,2’-bipyridine (reagent plus ⩾99%), ethylene glycol (99.9%), anhydrous diethyl ether (99.9%), hydrochloric acid (HCl; 37% w/v), 1—vinylimidazole (⩾99.9%), allylamine (AA, 98%), sodium dithionite (technical grade) and azobisisobutyronitrile (AIBN; 99%).

Calhoun M.C., Stachurski C.D., Winn S.L., Gizzie E.A., Daniel A.W., Schley N.D, & Cliffel D.E. (2022). Trace Oxygen Affects Osmium Redox Polymer Synthesis for Wired Enzymatic Biosensors. Journal of the Electrochemical Society, 169(1), 016506.

+ Open protocol

+ Expand

Collagenase-Based Tissue Dissociation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Collagenase type III (COLase3) (Clostridium histolyticum) was obtained from Worthington (Lakewood, NJ, USA). PNGaseF PRIME™ was obtained from N-Zyme Scientific (Doylestown, PA, USA). Sigma Aldrich (St. Louis, MO, USA) was the source used for acetonitrile, α-cyano-4-hydroxycinnamic acid (CHCA), trifluoroacetic acid (TFA), ammonium bicarbonate, ammonium phosphate monobasic, and calcium chloride. Dewaxing (xylenes, 200-proof ethanol, methanol) solvents and high-performance liquid chromatography (HPLC)-grade water were purchased from Fisher Scientific (Pittsburgh, PA, USA).

Rujchanarong D., Lefler J., Saunders J.E., Pippin S., Spruill L., Bethard J.R., Ball L.E., Mehta A.S., Drake R.R., Ostrowski M.C, & Angel P.M. (2021). Defining the Tumor Microenvironment by Integration of Immunohistochemistry and Extracellular Matrix Targeted Imaging Mass Spectrometry. Cancers, 13(17), 4419.

+ Open protocol

+ Expand

Stimulating E-selectin in HUVECs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human umbilical vein endothelial cells (HUVECs) were maintained as previously described.^{21 (link)} Spironolactone (Sigma) in dimethylsulfoxide (Sigma) and estrogen (Sigma) in 200-proof ethanol (Fisher) were diluted to 1μM and 10nM, respectively, in M199 media (Gibco) for application to cells. For stimulation of E-selectin, cells were pretreated with Spironolactone, estrogen, or appropriate vehicle(s) for 1 hour and then stimulated with low-dose TNFα (80pg/mL, Biolegend) for 20 hours. Otherwise, Spironolactone and estrogen were applied to cells for 20 hours. All samples contained the same concentration of DMSO and/or ethanol. Leukocyte adhesion and luciferase assays were performed as previously described.^{12 (link),21 (link)} Each cell treatment was carried out in triplicate, and each experiment was performed a minimum of three times.

Moss M.E., Lu Q., Iyer S.L., Engelbertsen D., Marzolla V., Caprio M., Lichtman A.H, & Jaffe I.Z. (2019). Endothelial Mineralocorticoid Receptors Contribute to Vascular Inflammation in Atherosclerosis in a Sex-Specific Manner. Arteriosclerosis, thrombosis, and vascular biology, 39(8), 1588-1601.

+ Open protocol

+ Expand

Ethanol Sedation Assay for Drosophila

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

A modified ethanol sedation assay from Maples and Rothenfluh was followed as previously reported [22 (link),23 (link)]. Male or female flies (8–10) were sedated using CO₂ and sorted into empty vials. Flies were allowed to recover from the effects of CO₂ anesthesia for 0.5–8 h before sedation assay. To determine time of sedation, the cotton plug of each vial was replaced with a new plug soaked with 500 μL of the appropriate dose (25%, 50%, 75%, and 100% v/v) of 200-proof ethanol (Fisher Scientific). After one minute, each vial was tapped once on the table to relocate the flies to the bottom of the vial. After ten seconds, the number of flies exhibiting loss-of-righting reflex were recorded. This procedure was repeated after every minute of ethanol exposure for each vial until at least half of the flies in the vial were sedated. Loss of righting reflex includes flies on their backs, stationary flies, stationary flies with rapid wing movement, and spinning in one location. The number of minutes it took for half the flies in the vial to sedate was recorded for each vial as the median sedation time (ST50). Linear interpolation was used to determine ST50 when there was an odd number of flies in the trial or when 50% sedation was reached in between intervals.

Tremblay S., Zeng Y., Yue A., Chabot K., Mynahan A., Desrochers S., Bridges S, & Ahmad S.T. (2022). Caffeine Delays Ethanol-Induced Sedation in Drosophila. Biology, 12(1), 63.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!