Antibodies for flow cytometry were purchased from eBioscience. Antibodies for Western blot against Srebp1, Srebp2, LXRs, and Sumo1, -2, and -3 were purchased from Abcam, and other Western blot antibodies were purchased from Cell Signaling Technology. Cytokines were purchased from R&D Systems, and neutralization antibodies were purchased from Bio X Cell unless otherwise indicated. Cholesterol, 24(S)-hydroxycholesterol, 25-hydroxycholesterol, 7β-hydroxycholesterol, 20α-hydroxycholesterol, 3β-Hydroxy-5-cholesten-7-one, 22(R)-hydroxycholesterol, 22(S)-hydroxycholesterol, 5β-Cholestan-3β-ol, 5α-Cholest-7-en-3β-ol, 5,24-Cholestadien-3β-ol, 5-α-cholestane, β-cyclodextrin, simvastatin, lovastatin, and 2-D08 were purchased from Sigma-Aldrich. T0 and GW3965 were purchased from Cayman Chemical. Overexpression plasmids were purchased from Addgene unless otherwise indicated. All experiments were conducted according to the manufacturer’s protocols unless otherwise indicated.
22 s hydroxycholesterol
Manufactured by Merck Group
Sourced in United Kingdom
22(S)-hydroxycholesterol is a pure chemical compound that can be used for laboratory research purposes. It is a naturally occurring oxysterol derived from cholesterol. The core function of this product is to serve as a reference standard or analytical tool for scientific investigations, without any interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Lovastatin, by Merck Group (3 mentions)
22 r hydroxycholesterol, by Merck Group (3 mentions)
25 hydroxycholesterol, by Merck Group (3 mentions)
Simvastatin, by Merck Group (2 mentions)
20α hydroxycholesterol, by Merck Group (2 mentions)
Cortisol, by Merck Group (2 mentions)
Gw4064, by Merck Group (2 mentions)
Gsk2033, by Merck Group (2 mentions)
7β hydroxycholesterol, by Merck Group (1 mentions)
β cyclodextrin, by Merck Group (1 mentions)
Antibodies for flow cytometry, by Thermo Fisher Scientific (1 mentions)
5α cholest 7 en 3β ol, by Merck Group (1 mentions)
Overexpression plasmids, by Addgene (1 mentions)
Gw3965, by Cayman Chemical (1 mentions)
5α cholestane, by Merck Group (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Gw3965, by Merck Group (1 mentions)
Anti srebp 1, by Santa Cruz Biotechnology (1 mentions)
Anti srebp2, by Abcam (1 mentions)
Fluvastatin, by Merck Group (1 mentions)
7 protocols using 22 s hydroxycholesterol
1
Cholesterol Metabolism Pathway Assays
2
Cholesterol Regulation Pathway Assay
3
Oxysterol and rhBMP-2 Combination for Bone Regeneration
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The oxysterol combination (SS) was prepared by mixing equal amounts of 100 μM 20S-hydroxycholesterol (Sigma-Aldrich) with 100 μM 22S-hydroxycholesterol (Sigma-Aldrich) dissolved in 1% DMSO in PBS. The other test agent, rhBMP-2, was diluted to a saturated concentration of 1.5 mg/ml with injectable saline. A 50-μl Hamilton syringe (no. 705; Hamilton Company, Reno, NV, USA) with a 32-gauge needle was used to administer an injection into the extraction sockets. Under general anesthesia, 10 μl of SS and 10 μl of rhBMP-2 were injected slowly into the extraction sockets of the rats in each group.
4
Bile Acid Regulation Assay
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Cortisol, cortisone, cholic acid (CA), chenodeoxycholic acid (CDCA), GW4064, 22(S)-Hydroxycholesterol and GSK2033 were purchased from Sigma-Aldrich (Dorset, UK). All cell treatments, including bile acid measurements, were performed in serum-free and phenol-red free media, containing 4.5 g/L glucose (Zen Bio Inc., Durham, NC, USA).
5
Steroid Compound Procurement for Research
6
Cultivating Liver and Kidney Cell Lines
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HepG2 cells (Cat#HB-8065) and HEK293 cells (Cat#CRL-11268) were purchased from ATCC. Huh7
cells were purchased from the Japanese Cancer Research Resources Bank (Cat#JCRB0403). All
cell lines were cultured in Dulbecco’s minimum essential medium (DMEM) (Thermo
Fisher Scientific), containing 4.5 g/L glucose and supplemented with 10% fetal bovine
serum, 1% penicillin/streptomycin and 1% non-essential amino acids (Thermo Fisher
Scientific).
Dexamethasone (500 nM), cortisol (500 nM), prednisolone (500 nM), GW4064 (5 μM),
GSK2033 (100 nM), 22(S)-hydroxycholesterol (10 μM) and RU486 (5 μM) were
purchased from Sigma-Aldrich. SPA70 (10 μM) was purchased from Axon Medchem
(Groningen, Netherlands). For all cell treatments, HEK293, HepG2 and Huh7 cells were
cultured in serum-free and phenol red-free media containing 4.5g/L glucose and
supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin and 1% non-essential
amino acids.
cells were purchased from the Japanese Cancer Research Resources Bank (Cat#JCRB0403). All
cell lines were cultured in Dulbecco’s minimum essential medium (DMEM) (Thermo
Fisher Scientific), containing 4.5 g/L glucose and supplemented with 10% fetal bovine
serum, 1% penicillin/streptomycin and 1% non-essential amino acids (Thermo Fisher
Scientific).
Dexamethasone (500 nM), cortisol (500 nM), prednisolone (500 nM), GW4064 (5 μM),
GSK2033 (100 nM), 22(S)-hydroxycholesterol (10 μM) and RU486 (5 μM) were
purchased from Sigma-Aldrich. SPA70 (10 μM) was purchased from Axon Medchem
(Groningen, Netherlands). For all cell treatments, HEK293, HepG2 and Huh7 cells were
cultured in serum-free and phenol red-free media containing 4.5g/L glucose and
supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin and 1% non-essential
amino acids.
7
Cholesterol Metabolite Modulation of Shh Signaling
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Water soluble SAG was purchased from Calbiochem. Shh-N was produced in bacteria according to (88 (link)). BM15.766 (Santa Cruz) and AY9944 (Calbiochem) were solubilized in Dimethyl sulfoxide (DMSO). BM15.766 was present at a concentration of 15 μm and AY9944 at 10 μm in assays unless stated otherwise. Lovastatin (Sigma) solubilized in H2O was present at a concentration of 5 μm in assays. 7DHC, 22(S)-hydroxyCholesterol and 20α-hydroxyCholesterol (all Sigma) were solubilized in ethanol. Cholesterol (Sigma) was solubilized in 30% 2-hydroxypropyl-β-cyclodextrin (Sigma). Cholesterol and 7DHC were present at 50 μm , and 22S- and 20α-hydroxyCholesterol were present at 10 μm in assays. In all cases equivalent concentrations of vehicle were added to controls. When present 2-hydroxypropyl-β-cyclodextrin was at a concentration of 0.1%.
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