Bard1

Manufactured by Fortis Life Sciences

Sourced in United States

The BARD1 is a laboratory instrument designed for protein analysis. It is used to detect and quantify specific proteins in biological samples. The core function of the BARD1 is to provide accurate and reliable protein measurement data to support scientific research and clinical applications.

Automatically generated - may contain errors

Lab products found in correlation

Brca1, by Santa Cruz Biotechnology (1 mentions) H3k9ac, by Merck Group (1 mentions) Brca1, by Abcam (1 mentions) Brca2, by Merck Group (1 mentions) Mms 101p, by New England Biolabs (1 mentions) Sc 2032, by Santa Cruz Biotechnology (1 mentions) Phospho rpa32 s4s8, by Fortis Life Sciences (1 mentions) Rad51, by Santa Cruz Biotechnology (1 mentions) Ecl kit, by Thermo Fisher Scientific (1 mentions)

2 protocols using bard1

Antibody Detection of DNA Repair Proteins

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The antibodies used were rabbit polyclonal antibodies to BRCA1 (C20; Santa Cruz Biotechnology, USA), BARD1 (BL518; Bethyl Laboratories, USA), H3K9ac (07-352; Millipore, Germany), H3K56ac (2134-1; EPITMICS, USA), H4ac (06-866; Millipore), 53BP1 (Novus Biologicals, USA) RIF1, (A300-569A; Bethyl Laboratories) and RAD51 (Bio Academia, Japan), and mouse monoclonal antibodies to H3K9me2 (CMA307; Millipore), γH2AX (JBW301; Millipore) and α-tubulin and β-tubulin (DMIA+BMIB; Neomarkers, USA).

Fukuda T., Wu W., Okada M., Maeda I., Kojima Y., Hayami R., Miyoshi Y., Tsugawa K.I, & Ohta T. (2015). Class I histone deacetylase inhibitors inhibit the retention of BRCA1 and 53BP1 at the site of DNA damage. Cancer Science, 106(8), 1050-1056.

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Western Blot Analysis of DNA Damage Response Proteins

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Protein was extracted from cells harvested two days after transfection with the indicated siRNAs using NETN buffer (20 mM Tris-HCl pH 8, 420 mM NaCl, 1 mM EDTA, 0.5% Igepal CA-630, 1 mM DTT, and Roche Protease Inhibitor Cocktail) and 8 freeze/thaw cycles. Blots (20–50 μg of total protein) were probed with the following antibodies: BARD1 (Bethyl, A300-263A; Santa Cruz Biotech, Sc11438), BRCA1 (Abcam, ab16780), 53BP1 (Abcam, ab36823), Flag M2-HRP (Sigma, A8592), Phospho RPA32 S4/S8 (Bethyl, A300-245A), BRCA2 (EMD Millipore, OP95-100UG), RAD51 (Santa Cruz Biotech, sc-8349), Actin (Abcam, ab3280), Tubulin (Santa Cruz Biotech, sc-53030), HA.11 (16B12) (Covance, MMS-101P), or GST-HRP (NEB, E2624S) according to the instructions provided by the manufacturers. If needed, the blots were incubated with HRP-conjugated secondary antibodies (Pierce 31450 for rabbit anti-mouse IgG-HRP; Sigma A6154 for goat anti-rabbit IgG-HRP; Santa Cruz Biotech Sc-2032 for goat anti-rat IgG-HRP) before visualization of protein signals using the ECL kit (Thermo Scientific Pierce).

Zhao W., Steinfeld J.B., Liang F., Chen X., Maranon D.G., Ma C.J., Kwon Y., Rao T., Wang W., Chen S., Song X., Deng Y., Jimenez-Sainz J., Lu L., Jensen R.B., Xiong Y., Kupfer G.M., Wiese C., Greene E.C, & Sung P. (2017). Promotion of RAD51-mediated homologous DNA pairing by BRCA1-BARD1. Nature, 550(7676), 360-365.

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