Multitest 6 color reagents

Manufactured by BD

Multitest 6-color reagents are a set of laboratory reagents designed for use in flow cytometry applications. The reagents contain a combination of six different fluorescent dyes that can be used to label and identify multiple cell populations or targets within a single sample.

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Lab products found in correlation

Lymphoprep, by Takeda Pharmaceuticals (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Cd4 percp cy5, by BD (1 mentions) Cd56 fitc, by BD (1 mentions) Cd20 apc, by BD (1 mentions) Cd8 percp cy5, by BD (1 mentions) Cd3 apc h7, by BD (1 mentions) Cd19 percp cy5, by BD (1 mentions) Cd20 apc, by BioLegend (1 mentions) Facscalibur, by BD (1 mentions) Canto 2, by BD (1 mentions) Cd4 pe cy7, by BD (1 mentions) Cd3 apc, by BD (1 mentions)

Close spelling variants of this product

BD Multitest 6-color TBNK Reagent Kit BD Multitest 6-color TBNK reagent MultiTest reagents BD Multitest

2 protocols using multitest 6 color reagents

Isolation and Characterization of PBMCs

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PBMCs were isolated by using standard density gradient centrifugation techniques with Lymphoprep (Nycomed, Oslo, Norway). Absolute numbers of lymphocytes, T cells, B cells, and natural killer cells were determined with Multitest 6-color reagents (BD Biosciences, San Jose, Calif), according to the manufacturer's instructions. For PBMC immunophenotyping, we refer to the Methods section in this article's Online Repository at www.jacionline.org.
PBMCs were resuspended in PBS at a concentration of 5 to 10 × 10⁶ cells/mL and labeled with 0.5 μmol/L carboxyfluorescein succinimidyl ester (CFSE; Molecular Probes, Eugene, Ore), as described previously³³ (link) and in the Methods section in this article's Online Repository, to analyze the ex vivo activation of T and B cells. Proliferation of B and T cells was assessed by measuring CFSE dilution in combination with the same mAbs used for immunophenotyping. Analysis of cells was performed with a FACSCanto II flow cytometer (BD Biosciences) and FlowJo software (TreeStar, Ashland, Ore). Patient samples were analyzed simultaneously with PBMCs from healthy control subjects.

Tuijnenburg P., Lango Allen H., Burns S.O., Greene D., Jansen M.H., Staples E., Stephens J., Carss K.J., Biasci D., Baxendale H., Thomas M., Chandra A., Kiani-Alikhan S., Longhurst H.J., Seneviratne S.L., Oksenhendler E., Simeoni I., de Bree G.J., Tool A.T., van Leeuwen E.M., Ebberink E.H., Meijer A.B., Tuna S., Whitehorn D., Brown M., Turro E., Thrasher A.J., Smith K.G., Thaventhiran J.E, & Kuijpers T.W. (2018). Loss-of-function nuclear factor κB subunit 1 (NFKB1) variants are the most common monogenic cause of common variable immunodeficiency in Europeans. The Journal of Allergy and Clinical Immunology, 142(4), 1285-1296.

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Flow Cytometry Analysis of Blood Cells

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Absolute numbers of thrombocytes and ferritin levels were routinely measured according to the established diagnostic guidelines. Absolute numbers of lymphocytes were determined with Multitest 6-color reagents (BD Biosciences, San Jose, Calif) according to the manufacturer's instructions. For additional flow cytometry, PBMCs were resuspended in PBS containing 0.5% (wt/vol) BSA and 0.01% sodium azide and then incubated with saturating concentrations of fluorescently labeled conjugated mAb antibodies. Patient samples were analyzed simultaneously with PBMCs from healthy donors. The following directly conjugated mAbs were used: CD3-APC, CD3-APC-H7, CD4-PE-Cy7, CD4-PerCP-Cy5.5, CD8-PerCP-Cy5.5, CD19-PerCP-Cy5.5, CD20-APC, and CD56-FITC (from BD Biosciences); IgD-PE and gamma-1 isotype (from BD Pharmingen, San Diego, Calif); CD20-APC (from Biolegend San Diego, Calif); CD16-FITC and CD27-FITC (from Sanquin, Amsterdam, The Netherlands); and CD45RA-PE (RD-1) (from Beckman Coulter, Brea, Calif). Analysis of cells was performed using a FACS Calibur or Canto-II flow cytometer (from BD Biosciences) and FlowJo software.

Verhoeven D., Schonenberg-Meinema D., Ebstein F., Papendorf J.J., Baars P.A., van Leeuwen E.M.M., Jansen M.H., Lankester A.C., van der Burg M., Florquin S., Maas S.M., van Koningsbruggen S., Krüger E., van den Berg J.M, & Kuijpers T.W. (2022). Hematopoietic stem cell transplantation in a patient with proteasome-associated autoinflammatory syndrome (PRAAS). The Journal of allergy and clinical immunology, 149(3).

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