Log In Sign up
The largest database of trusted experimental protocols

Rat isotype control

Manufactured by Merck Group
Visit Supplier

The Rat Isotype Control is a laboratory reagent used to establish background staining levels in flow cytometry experiments involving rat-derived samples. It serves as a reference point to distinguish specific antibody binding from non-specific binding. The Rat Isotype Control is a crucial tool for accurate data analysis and interpretation in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Isotype control (8 citations) Rat IgG isotype control (5 citations)

2 protocols using rat isotype control

1

Antibody Depletion of CD8+ T Cells in MmuPV1-Infected Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
SKH-1 mice were infected with MmuPV1 or sham infected with MmuPV1
virus-like particles (VLPs: L1Met3019 , 105 μg in 40 μL PBS per mouse) applied
to their abraded back skin. Four weeks later, MmuPV1-infected immune mice and
sham(VLP)-infected controls were started on anti-CD8 (Rat Anti-Mouse
CD8⍺, YTS 169.4, BioXCell, West Lebanon, NH) or IgG (Rat Isotype Control,
Sigma-Aldrich) antibody treatment at 750 μg in 200 μL sterile PBS
(first dose) followed by 250 μg in 200 μL sterile PBS weekly
intraperitoneal injections (Extended Data Fig.
5g and Supplementary Table 4a). One day after the first antibody treatment,
mice underwent UV carcinogenesis protocol as described above.
Strickley J.D., Messerschmidt J.L., Awad M.E., Li T., Hasegawa T., Ha D.T., Nabeta H.W., Bevins P.A., Ngo K.H., Asgari M.M., Nazarian R.M., Neel V.A., Jenson A.B., Joh J, & Demehri S. (2019). Immunity to Commensal Papillomaviruses protects against Skin Cancer. Nature, 575(7783), 519-522.
+ Open protocol
+ Expand
2

Antibody Depletion of CD8+ T Cells in MmuPV1-Infected Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
SKH-1 mice were infected with MmuPV1 or sham infected with MmuPV1
virus-like particles (VLPs: L1Met3019 , 105 μg in 40 μL PBS per mouse) applied
to their abraded back skin. Four weeks later, MmuPV1-infected immune mice and
sham(VLP)-infected controls were started on anti-CD8 (Rat Anti-Mouse
CD8⍺, YTS 169.4, BioXCell, West Lebanon, NH) or IgG (Rat Isotype Control,
Sigma-Aldrich) antibody treatment at 750 μg in 200 μL sterile PBS
(first dose) followed by 250 μg in 200 μL sterile PBS weekly
intraperitoneal injections (Extended Data Fig.
5g and Supplementary Table 4a). One day after the first antibody treatment,
mice underwent UV carcinogenesis protocol as described above.
Strickley J.D., Messerschmidt J.L., Awad M.E., Li T., Hasegawa T., Ha D.T., Nabeta H.W., Bevins P.A., Ngo K.H., Asgari M.M., Nazarian R.M., Neel V.A., Jenson A.B., Joh J, & Demehri S. (2019). Immunity to Commensal Papillomaviruses protects against Skin Cancer. Nature, 575(7783), 519-522.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!