On day 24, mice from each group were euthanized. The lungs and livers were harvested, and the tumor metastatic lesions in the lung were counted. Then, the lung tissues were processed and stained with H&E. Moreover, the distribution of oncolytic adenoviruses in the lung was analyzed by immunohistochemistry using a mouse anti-adenovirus antibody (Abcam, Cambridge, MA) and a goat anti-human decorin antibody (R&D Systems, Minneapolis, MN). Furthermore, the infiltration of lymphocytes, including CD3+ T lymphocytes and macrophages, was also detected by immunohistochemistry using rabbit anti-mouse CD3 (Abcam, Cambridge, MA) and rabbit anti-mouse CD68 (Servicebio, Wuhan, China), respectively. Horseradish peroxidase (HRP)-conjugated rabbit anti-goat immunoglobulin G (IgG) (Servicebio, Wuhan, China) or HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (Servicebio, Wuhan, China).
Goat anti human decorin antibody
Manufactured by R&D Systems
Goat anti-human decorin antibody is a laboratory reagent used for the detection and quantification of decorin, a proteoglycan found in the extracellular matrix. This antibody is produced in goats and specifically targets the human decorin protein.
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Lab products found in correlation
2 protocols using goat anti human decorin antibody
1
Evaluation of Oncolytic Adenovirus Therapy
2
Decorin and Cell Proliferation Regulation
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Human recombinant decorin was purchased from R&D Systems (Minneapolis, MN, USA). The rabbit monoclonal anti-human decorin antibody (product code ab151988) used for immunoblotting and immunohistochemistry (IHC) and the mouse monoclonal anti-human β-actin antibody used for immunoblotting were purchased from Abcam (Cambridge, MA, USA). The mouse monoclonal anti-human p21 antibody (product code 554228) used for immunoblotting was purchased from BD Biosciences (San Jose, CA, USA). The rabbit monoclonal anti-human c-Met antibody (product code LS-C49950) used for immunoblotting and IHC was purchased from LifeSpan Biosciences, Inc. (Seattle, WA, USA). A goat anti-human decorin antibody used as a neutralizing antibody for decorin was purchased from R&D Systems. The BD Falcon 96-well microplates used for the cell proliferation assays were purchased from BD (Franklin Lakes, NJ, USA). A rabbit anti-progesterone receptor (PR) antibody (H-190) used in the chromatin immunoprecipitation (ChIP) assay was purchased from Santa Cruz Biotechnology.
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