Milli q iq 7005 water purification system

Manufactured by Merck Group

Sourced in United States

The Milli-Q® IQ 7005 Water Purification System is a compact, versatile water purification system designed to produce ultrapure water for a variety of laboratory applications. The system utilizes a multi-stage purification process to remove contaminants and produce high-quality water with low levels of total organic carbon, bacteria, and endotoxins.

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4 protocols using milli q iq 7005 water purification system

Chitosan Characterization and Application

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Low molecular weight CS (LMW) with 50–190 kDa molecular weight and 75–85% deacetylation degree, medium molecular weight CS (MMW) with 190–310 kDa molecular weight and 75–85% deacetylation degree, and high molecular weight CS (HMW) with 310–390 kDa molecular weight and above 75% deacetylation degree were purchased from Merck (Darmstadt, Germany). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), streptomycin, and penicillin were purchased from Sigma-Aldrich, St. Louis, MO, USA. IPA was purchased from POCH (Gliwice, Poland). MilliQ water was used to prepare all the aqueous solutions (Milli-Q^® IQ 7005 Water Purification System, Millipore, Boston, MA, USA). All other reagents were of analytical grade or higher. Chloroform, hydroxyacetic acid, and sodium hydroxide were purchased from Avantor Performance Materials Poland S. A. (Gliwice, Poland). The carbon dioxide used to saturate the CS precipitate was obtained from Linde Gaz Polska Sp. z o. o. (Gdańsk, Poland). Tryptic soy agar (TSA) medium was purchased from Merck (Darmstadt, Germany).

Banach-Kopeć A., Mania S., Pilch J., Augustin E., Gabriel I, & Tylingo R. (2022). A Novel Method of Endotoxins Removal from Chitosan Hydrogel as a Potential Bioink Component Obtained by CO2 Saturation. International Journal of Molecular Sciences, 23(10), 5505.

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Synthesis and Characterization of Functionalized Biomaterials

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First, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC × HCl), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), β-cyclodextrin (β-CD), 4-dimethylaminopyridine (DMAP), Amiloride, Cytochalasin D, dimethyl sulfoxide (DMSO), Dynasore, folic acid (FA), Filipin III, Pitstop 2, streptomycin, and penicillin were purchased from Sigma-Aldrich, St. Louis, MO, USA. MilliQ water was used for the preparation of all aqueous solutions (Milli-Q^® IQ 7005 Water Purification System, Millipore, Billerica, MA, USA). All reagents and chemicals used in experiments were of analytical grade and were used as received.

Pilch J., Kowalik P., Kowalczyk A., Bujak P., Kasprzak A., Paluszkiewicz E., Augustin E, & Nowicka A.M. (2022). Foliate-Targeting Quantum Dots-β-Cyclodextrin Nanocarrier for Efficient Delivery of Unsymmetrical Bisacridines to Lung and Prostate Cancer Cells. International Journal of Molecular Sciences, 23(3), 1261.

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Identification of Undeclared Pharmaceutical Adulterants

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A sample with oval beige tablet form (200mg/tablet) was submitted for testing by a consumer who was skeptical on its claim of “pure nature”. Vardenafil (VAR) and desethylvardenafil (DEV) as reference chemicals were purchased from Alta Scientific (Tianjin, China). LC-MS grade methanol, acetonitrile (ACN), formic acid, ammonium hydroxide (NH₄OH) and GC-grade dichloromethane (DCM) were purchased from Fisher Scientific (New Jersey, USA). Deuterated chloroform (CDCl₃) was purchased from Merck (Darmstadt, Germany). AR (analytical reagent)-grade chemicals and reagents were purchased from local commercial suppliers. The lab water (ultra-pure grade with 18.2 MΩ) was prepared by a Milli-Q IQ7005 water purification system (Millipore SAS, Molsheim, France).

Li C., Wang X., Wu S., Zhao J., Fang J., Li H., Zhu Y., Zhang K., Peng J., Mao J., Li W., He K., Wang N, & Dong F. (2023). Separation and structural elucidation of a novel vardenafil analogue as an adulterant in a natural health care dietary supplement. Heliyon, 9(4), e15418.

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Fibroblast Cell Culture Protocol

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Poly(vinyl alcohol) (PVA), Poly(ε-caprolactone) (PCL), Agar-Agar (powder BioScence–Grade, ROTH, Nuremberg, Germany), Standard agarose (powder, ROTH, Nuremberg, Germany), Fluorescein Isothiocyanate-Dextran (wt. 10,000, Sigma-Aldrich, St. Louis, MO, USA) (FSC), H3PO4, and Glyoxal (Sigma-Aldrich, MO, USA) were used. In vitro experiments were performed on immortalized BALB/3T3 mouse fibroblast cell lines obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Mouse embryo fibroblast BALB/3T3 clone A31 cells (ATCC CCL-163) were cultured as adherent monolayers in plastic Petri dishes Nunc™ (Fisher Scientific, Waltham, MA, USA) in Dulbecco’s modified eagle medium (4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate, Sigma-Aldrich) supplemented with 10% (v/v) fetal bovine serum (Sigma-Aldrich, MO, USA) and antibiotic–antimycotic solution (Sigma-Aldrich, MO, USA). MilliQ water was used for the preparation of all aqueous solutions (Milli-Q^® IQ 7005 Water Purification System, Millipore, Billerica, MA, USA). All reagents and chemicals used in experiments were of analytical grade and were used as received.

Bocková M., Pashchenko A., Stuchlíková S., Kalábová H., Divín R., Novotný P., Kestlerová A., Jelen K., Kubový P., Firment P., Fedačko J., Jarošíková T., Rulc J., Rosina J., Nečas A., Amler E, & Hoch J. (2022). Low Concentrated Fractionalized Nanofibers as Suitable Fillers for Optimization of Structural–Functional Parameters of Dead Space Gel Implants after Rectal Extirpation. Gels, 8(3), 158.

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