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Pcmv6 sphk1

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PCMV6-SPHK1 is a plasmid vector containing the open reading frame (ORF) of the human sphingosine kinase 1 (SPHK1) gene. The gene is under the control of the cytomegalovirus (CMV) promoter, which allows for the expression of the SPHK1 protein in various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Puseamp containing a myc tag, by Merck Group (2 mentions) Pcdna3.1 s1pr1, by OriGene (2 mentions) Lipofectamine, by Thermo Fisher Scientific (2 mentions) Nucleofection, by Lonza (2 mentions)

2 protocols using pcmv6 sphk1

1

Molecular Manipulation of B Cell Signaling

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GC B cells were nucleofected with pcDNA3.1-PRDM1 or empty vector, cultivated and enriched as before.20 (link), 21 (link) HL cell lines and DG75 cells were transfected by Nucleofection (Lonza, Basel, Switzerland) and HEK293 cells were transfected by Lipofectamine (ThermoFisher Scientific) using the following vectors and corresponding controls: pcDNA3.1-S1PR2-HA (UMR cDNA Resource Center University of Missouri-Rolla, Rolla, MO, USA), pSG5-myc-p110α-CAAX (Julian Downward, Addgene, Cambridge, MA, USA), Akt1/PKBα cDNA (activated) in pUSEamp containing a MYC tag (Merck Millipore, Billerica, MA, USA), pcDNA3.1- S1PR1 (Dr Ian Paterson, University of Malaya, Kuala Lumpur, Malaysia) and pCMV6-SPHK1 (Origene technologies, Rockville, MD, USA).
Vrzalikova K., Ibrahim M., Vockerodt M., Perry T., Margielewska S., Lupino L., Nagy E., Soilleux E., Liebelt D., Hollows R., Last A., Reynolds G., Abdullah M., Curley H., Care M., Krappmann D., Tooze R., Allegood J., Spiegel S., Wei W., Woodman C.B, & Murray P.G. (2017). S1PR1 drives a feedforward signalling loop to regulate BATF3 and the transcriptional programme of Hodgkin lymphoma cells. Leukemia, 32(1), 214-223.
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2

Genetic Manipulation of Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
GC B cells were nucleofected with pcDNA3.1-PRDM1 or empty vector, cultivated and enriched as before.20 (link),21 (link) HL cell lines and DG75 cells were transfected by nucleofection (Lonza, Basel, Switzerland) and HEK293 transfected by lipofectamine (ThermoFisher Scientific) using the following vectors and corresponding controls: pcDNA3.1-S1PR2-HA (UMR cDNA Resource Center University of Missouri-Rolla, MO, USA), pSG5-myc-p110α-CAAX (Julian Downward, Addgene, Cambridge, MA, USA), Akt1/PKBα cDNA (activated) in pUSEamp containing a MYC tag (Merck Millipore, Billerica, MA, USA), pcDNA3.1-S1PR1 (Dr Ian Paterson, University of Malaya) and pCMV6-SPHK1 (Origene technologies, Rockville, MD, USA).
Vrzalikova K., Ibrahim M., Vockerodt M., Perry T., Margielewska S., Lupino L., Nagy E., Soilleux E., Liebelt D., Hollows R., Last A., Reynolds G., Abdullah M., Curley H., Care M., Krappmann D., Tooze R., Allegood J., Spiegel S., Wei W., Woodman C.B, & Murray P.G. (2017). S1PR1 drives a feedforward signalling loop to regulate BATF3 and the transcriptional programme of Hodgkin lymphoma cells. Leukemia, 32(1), 214-223.
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