Bcip nbt staining

Manufactured by Vector Laboratories

Sourced in Switzerland

BCIP/NBT staining is a chromogenic substrate system used for the detection and visualization of alkaline phosphatase (AP) activity in various biological applications.

Automatically generated - may contain errors

Lab products found in correlation

Sp6 polymerase, by Thermo Fisher Scientific (1 mentions) T3 or t7 polymerase, by Roche (1 mentions) T3 or t7 polymerase, by Thermo Fisher Scientific (1 mentions) Anti digoxigenin fab fragment antibody, by Roche (1 mentions)

4 protocols using bcip nbt staining

Whole-mount in situ hybridization protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

WISH was performed as described previously (Thisse and Thisse, 2008 (link)). Digoxigenin-labeled RNA probes were transcribed with T7, T3, or SP6 polymerase (Ambion). The probes were detected using alkaline phosphatase-coupled anti-digoxigenin Fab fragment antibodies (Roche) with BCIP/NBT staining (Vector Laboratories). The stained embryos were then photographed using a stereomicroscope (Nikon) equipped with a digital camera.

Deng Y., Han X., Chen H., Zhao C., Chen Y., Zhou J., de The H., Zhu J, & Yuan H. (2023). Ypel5 regulates liver development and function in zebrafish. Journal of Molecular Cell Biology, 15(3), mjad019.

+ Open protocol

+ Expand

Whole-mount mRNA in situ Hybridization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Digoxigenin-labeled antisense RNA probes were transcribed from linearized constructs using T3 or T7 polymerase (Roche). Whole-mount mRNA in situ hybridization was performed as described previously9 (link). The probes were detected using alkaline phosphatase (AP)-coupled anti-digoxigenin Fab fragment antibody (Roche) with BCIP/NBT staining (Vector Laboratories).

Yuan H., Zhang T., Liu X., Deng M., Zhang W., Wen Z., Chen S., Chen Z., de The H., Zhou J, & Zhu J. (2015). Sumoylation of CCAAT/enhancer-binding protein α is implicated in hematopoietic stem/progenitor cell development through regulating runx1 in zebrafish. Scientific Reports, 5, 9011.

+ Open protocol

+ Expand

Whole-mount mRNA In Situ Hybridization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Digoxigenin-labeled antisense RNA probes were transcribed from linearized constructs using T3, T7
or Sp6 polymerase (Roche). Whole-mount mRNA in situ hybridization was performed as described
previously18 (link). The probes were detected using alkaline phosphatase (AP)-coupled
anti-digoxigenin Fab fragment antibody (Roche) with BCIP/NBT staining (Vector Laboratories). The
probes used in this study included: cebpa, lfabp, trypsin, insulin,
fabp2, hhex, prox1 and foxa3.

Yuan H., Wen B., Liu X., Gao C., Yang R., Wang L., Chen S., Chen Z., de The H., Zhou J, & Zhu J. (2015). CCAAT/enhancer-binding protein α is required for hepatic outgrowth via the p53 pathway in zebrafish. Scientific Reports, 5, 15838.

+ Open protocol

+ Expand

Whole-mount mRNA In Situ Hybridization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Digoxigenin (DIG)-labeled RNA probes were synthesized with T3 or T7 polymerase (Ambion, Life Technologies, Carlsbad, CA, USA), using linearized cDNA plamid constructs. Whole-mount mRNA in situ hybridization was performed as described previously [57 (link)]. The DIG-labeled probes were detected using alkaline phosphatase-coupled anti-digoxigenin Fab fragment antibody (Roche, Basel, Switzerland) with BCIP/NBT staining (Vector Laboratories, Burlingame, CA, USA).

Liu X., Jia X., Yuan H., Ma K., Chen Y., Jin Y., Deng M., Pan W., Chen S., Chen Z., de The H., Zon L.I., Zhou Y., Zhou J, & Zhu J. (2015). DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish. Journal of Hematology & Oncology, 8, 15.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!