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Nuclear fast red and haematoxylin

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Nuclear fast red and haematoxylin are laboratory stains used in histology and cytology. Nuclear fast red is a red stain that binds to nucleic acids, while haematoxylin is a blue-purple stain that binds to basophilic structures. These stains are commonly used together to provide contrast and highlight cellular features during microscopic examination of biological samples.

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Haematoxylin (217 citations) Nuclear Fast Red (216 citations) Fast Red (79 citations)

2 protocols using nuclear fast red and haematoxylin

1

Histological Analysis of Colonic Biopsies

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Historic routine haematoxylin and eosin histology sections from the patient identified to harbor the NOX1 p.N122H mutation and IBD controls were analyzed. Staining of goblet cells or the proliferation marker Ki67 was performed on archived paraffin-embedded colonic epithelial biopsies. Specimens were stained using an Alcian blue and periodic acid-Schiff’s reagent staining kit (Clin-Tech Limited, UK) or rabbit monoclonal antibody to Ki67 (clone SP66, AbCam, Cambridge, USA), respectively. Nuclear fast red and haematoxylin (Sigma-Aldrich, Gillingham, UK) were used to counterstain cell nuclei.
Schwerd T., Bryant R.V., Pandey S., Capitani M., Meran L., Cazier J.B., Jung J., Mondal K., Parkes M., Mathew C., Fiedler K., McCarthy D.J., Sullivan P., Rodrigues A., Travis S., Moore C., Sambrook J., Ouwehand W.H., Roberts D.J., Danesh J., Russell R.K., Wilson D.C., Kelsen J.R., Cornall R., Denson L.A., Kugathasan S., Knaus U.G., Goncalves Serra E., Anderson C.A., Duerr R.H., McGovern D.P., Cho J., Powrie F., Li V.S., Muise A.M, & Uhlig H.H. (2017). NOX1 loss-of-function genetic variants in patients with inflammatory bowel disease. Mucosal immunology, 11(2), 562-574.
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2

Histological Analysis of Colonic Biopsies

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Historic routine haematoxylin and eosin histology sections from the patient identified to harbor the NOX1 p.N122H mutation and IBD controls were analyzed. Staining of goblet cells or the proliferation marker Ki67 was performed on archived paraffin-embedded colonic epithelial biopsies. Specimens were stained using an Alcian blue and periodic acid-Schiff’s reagent staining kit (Clin-Tech Limited, UK) or rabbit monoclonal antibody to Ki67 (clone SP66, AbCam, Cambridge, USA), respectively. Nuclear fast red and haematoxylin (Sigma-Aldrich, Gillingham, UK) were used to counterstain cell nuclei.
Schwerd T., Bryant R.V., Pandey S., Capitani M., Meran L., Cazier J.B., Jung J., Mondal K., Parkes M., Mathew C., Fiedler K., McCarthy D.J., Sullivan P., Rodrigues A., Travis S., Moore C., Sambrook J., Ouwehand W.H., Roberts D.J., Danesh J., Russell R.K., Wilson D.C., Kelsen J.R., Cornall R., Denson L.A., Kugathasan S., Knaus U.G., Goncalves Serra E., Anderson C.A., Duerr R.H., McGovern D.P., Cho J., Powrie F., Li V.S., Muise A.M, & Uhlig H.H. (2017). NOX1 loss-of-function genetic variants in patients with inflammatory bowel disease. Mucosal immunology, 11(2), 562-574.
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