Flutax 1

The fluorescent substrates BODIPY (BD)-verapamil, BODIPY-3-propionyl ethylenediamine hydrochloride (BD-EDA), BD-prazosin, and tetramethylrhodamine ethyl ester perchlorate (TMRE) were purchased from Serateh Biotech (Eugene, OR, USA). Other fluorescent compounds including 3-quinolinium 6-(dimethylamino)-2-[4-[4-(dimethylamino)-phenyl]-1,3-butadienyl]- 1-ethyl perchlorate (LDS-751), SYTO13, rhodamine 123 (Rh 123), calcein-acetoxymethyl (Cal-AM), Rhod-2-AM, rhodamine-6G (R6G), X-Rhod-1-AM, and rhodamine B hexyl ester (R6) were purchased from Invitrogen/Thermo Fisher (Carlsbad, CA, USA). Flutax-1 was purchased from Tocris Biosciences (Minneapolis, MN, USA). Cyclosporine A was purchased from the Alexis Corporation (Lausen, Switzerland). [N-ε(4-nitro-benzofurazan-7-yl)-D-Lys(8)]-cyclosporine A (NBD-CsA) was generously provided by Drs Anika Hartz and Bjoern Bauer, University of Kentucky (Lexington, KY, USA). All remaining chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA). The P-gp-specific monoclonal antibody C219 was provided by Fujirebio Diagnostic Inc. (Malvern, PA, USA), the MRK16 antibody was purchased from Kyowa Medex Company (Tokyo, Japan), and UIC2 antibody was purified from hybridoma cells as described in [28 (link)]. FITC-labeled anti-mouse secondary antibody IgG2a κ isotype was obtained from BD Biosciences (San Jose, CA, USA).