Feg 250

Scanning electron microscopy (SEM) was performed using FEI FEG250 with resolution of 1.2 nm equipped with SE and BSE detector. The surface morphology of the diamond and MoS₂ films was probed by atomic force microscopy (AFM) in a tapping mode (Bruker, Dimension Edge), using an etched silicon probe (Bruker, RTESPA - 300).
The crystallographic structure and orientation of the films were examined by X-ray diffraction (XRD) (CuKα) in the classical Bragg–Brentano and in the grazing-incidence configuration using BRUKER AXS D8 DISCOVER diffractometer with a rotating Cu anode.
The grazing-incidence wide-angle X-ray scattering (GIWAXS) measurements were performed using Nanostar system (Bruker AXS, Germany) equipped with IμS microfocus Cu X-ray source (λ = 0.154 nm). The parallel X-ray beam after Montel optics was further collimated using evacuated pinhole collimator equipped with two 550 µm pinholes separated by 1 m. The grazing-angle of incidence of X-ray beam on the sample was set to 0.8°. Reciprocal space maps were measured using an image plate detector at a sample-to-detector distance of 80 mm. All GIWAXS measurements were performed in fully evacuated chamber.

The sizes and shape of TDI droplets, the microspheres PPM and Pd@PPM were examined under optical microscope (OM, BX-51, Olympus). Their size (D_n) and size distribution (D_w/D_n) were obtained by counting at least 200 microspheres. Surface and inner morphology of the microspheres were observed under scanning electronic microscope (SEM, Quanta FEG-250, FEI). Their porous property was examined by BET (Nora 200E, Quantachrome) and mercury intrusion porosimetry (AutoPore IV 9500, Micromeritics). In addition, Pd@PPM was also examined using Inductive Coupled Plasma Optical Emission Spectrometer (ICP-OES, Optima 5300DV, Perkin-Elmer) and EDS (X-Max50, Oxford) analysis to affirm the presence of Pd. Temperature-programmed reduction (TPR) and chemisorption were performed using AutoChem II 2920 for the measurement of Pd dispersion. Powder X-ray diffraction (XRD) was done on a diffractometer (D8 Focus, Bruker).

WT and homozygous eyes were prepared for serial block face scanning electron microscopy (SBF-SEM) as previously described (43 (link),44 (link)). Eyes were oriented so the CZ to lens capsule was imaged in longitudinal cross section. Regions of interest were selected and scans taken using a Quanta Field emission gun (FEG) 250 (FEI) equipped with a Gatan 3View ultramicrotome at 3.8 kV collecting back scattered electrons. Data sets of 400 slices were collected and sections of ∼100 nm in thickness were removed from the sample blocks after each scan.