LPS (Escherichia coli serotype0111:B4), heptadecanoic acid (purity ≥98%), an internal standard (IS), and N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (BSTFA+1% TMCS; v/v) were purchased from Sigma–Aldrich (Saint Louis, MO, USA). O-Methylhydroxylamine hydrochloride (98.0% purity) was obtained from J&K Scientific Ltd (Beijing, China). Pyridine was obtained from Shanghai Macklin Biochemical (Shanghai, China). Chromatographic-grade methanol was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Water was purchased from Hangzhou Wahaha Company (Hangzhou, China).
O methylhydroxylamine hydrochloride
Manufactured by J&K Scientific
Sourced in China
O-methylhydroxylamine hydrochloride is a chemical compound that can be used as a laboratory reagent. It is a white crystalline solid that is soluble in water and other polar solvents. The compound is commonly used in organic synthesis and as a derivatizing agent for the analysis of carbonyl compounds.
Automatically generated - may contain errors
Lab products found in correlation
Pyridine, by Maclin Biochemical Technology (11 mentions)
Heptadecanoic acid, by Merck Group (7 mentions)
Chromatographic grade methanol, by Thermo Fisher Scientific (7 mentions)
Heptadecanoic acid, by Maclin Biochemical Technology (4 mentions)
Methanol, by Maclin Biochemical Technology (3 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Nonadecanoic acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Pyridine, by J&K Scientific (1 mentions)
Trimethylchlorosilane, by J&K Scientific (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Vancomycin, by Maclin Biochemical Technology (1 mentions)
7890b gc system, by Agilent Technologies (1 mentions)
Kz 2, by Wuhan Servicebio Technology (1 mentions)
Hp 5ms fused silica capillary column, by Agilent Technologies (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
13 protocols using o methylhydroxylamine hydrochloride
1
Metabolomic Analysis of LPS-Treated Cells
2
Quantification of Fructose Compounds
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High-fructose corn syrup (F55) was supplied by Guangzhou Shuangqiao Co., Ltd. (Guanzhou, China). Nonadecanoic acid was purchased from Sigma-Aldrich (Sigma, San Diego, USA). Methanol, hexane, and chloroform were of HPLC (High Performance Liquid Chromatography) grade and were from Merck (Darmstadt, German). Pyridine, O-methylhydroxylamine hydrochloride, N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA), and trimethylchlorosilane (TMCS) were from J&K Scientific Co. Ltd. (Tianjing, China).
3
Quantitative Metabolomic Analysis of MTX
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MTX (purity 99%), heptadecanoic acid (purity ≥ 98%), methanol (chromatographic grade), and pyridine were purchased from Macklin Biochemical (Shanghai, China). O-methylhydroxylamine hydrochloride (purity ≥ 98%) was obtained from J&K Scientific (Beijing, China). N, O-bis(trimethylsilyl) trifluoroacetamide (containing 1% trimethylchlorosilane) was obtained from Sigma Aldrich (St. Louis, MO, USA). Purified water was purchased from Wahaha (Hangzhou, China). All experimental procedures were conducted in compliance with the Guidelines for the Use of Laboratory Animals, and approved by the Ethical Committee for Animal Experimentation of the Affiliated Hospital of Jining Medical University (approval number: 2022B070).
4
Fatty Acid Quantification Protocol
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Tac was purchased from MedChemExpress (Shanghai, China). Heptadecanoic acid (purity: ≥98%; lot: SLBX4162), an internal standard (IS), and N,O-bis- (trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane (BSTFA + 1% TMCS; v/v; lot: BCBZ4865) were acquired from Sigma-Aldrich (Saint Louis, MO, USA). o-Methyl hydroxylamine hydrochloride (purity: 98.0%; lot: LG10T16) was obtained from J&K Scientific Ltd. (Beijing, China). Pyridine (lot: C10551455) was purchased from Shanghai Macklin Biochemical (Shanghai, China). Chromatographic-grade methanol was acquired from Thermo Fisher Scientific (Waltham, MA, USA), and water from Hangzhou Wahaha Company (Hangzhou, China).
5
Quantitative Metabolite Analysis Protocol
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DSS was purchased from MP Biomedicals (Beijing, China). N, O-bis(trimethylsilyl)trifluoroacetamide (with 1% trimethylchlorosilane; v/v; lot no. B-023), and heptadecanoic acid (as an internal standard, IS, purity ≥ 98%; lot no.H3500) were purchased from Sigma-Aldrich (Saint Louis, USA). O-methylhydroxylamine hydrochloride (purity: 98.0%; lot no. 542171) was purchased from J&K Scientific Ltd. (Beijing, China). Pyridine (lot no. C10486013) was obtained from Macklin Biochemical (Shanghai, China). Chromatographic-grade methanol was purchased from Thermo Fisher Scientific (Waltham, USA). Pure water was obtained from Wahaha Company (Hangzhou, China).
6
Vancomycin Quantification Protocol
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Vancomycin (USP ≥950 μg/mg), heptadecanoic acid (purity ≥98%), methanol (chromatographic grade) and pyridine were obtained from Shanghai Macklin Biochemical (Shanghai, China). O-methylhydroxylamine hydrochloride (purity ≥98%) was purchased from J&K Scientific Ltd. (Beijing, China). N, O-bis(trimethylsilyl)trifluoroacetamide (containing 1% trimethylchlorosilane) was obtained from Sigma Aldrich (St. Louis, MO. USA). The purified water was purchased from Hangzhou Wahaha Company (Hangzhou, China).
7
Heptadecanoic Acid-based Metabolite Analysis
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Heptadecanoic acid (purity: ≥98%; lot: SLBX4162), which was used as an internal standard (IS), and N, O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane (BSTFA + 1% TMCS; v/v; lot: BCBZ4865) were purchased from Sigma–Aldrich (Saint Louis, MO, USA). Chromatographic-grade methanol was purchased from Thermo Fisher Scientific (Waltham, MA, USA). o-Methyl hydroxylamine hydrochloride (purity: 98.0%; lot: 542171) was purchased from J&K Scientific Ltd., (Beijing, China). Water was purchased from Hangzhou Wahaha Company (Hangzhou, China). Pyridine (lot: C10486013) was purchased from Shanghai Macklin Biochemical (Shanghai, China).
A high-speed tissue homogenizer (KZ-II) was purchased from Servicebio (Wuhan, China). 7890B GC system equipped with a 7000 C mass spectrometer and the separation was on an HP-5MS fused-silica capillary column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies, USA).
A high-speed tissue homogenizer (KZ-II) was purchased from Servicebio (Wuhan, China). 7890B GC system equipped with a 7000 C mass spectrometer and the separation was on an HP-5MS fused-silica capillary column (30 m × 0.25 mm × 0.25 μm; Agilent Technologies, USA).
8
VPA Quantification via GC-MS
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VPA (purity≥98%) was obtained from Shanghai Macklin Biochemical (Shanghai, China). Heptadecanoic acid (purity≥98%) was obtained from Shanghai Macklin Biochemical (Shanghai, China) and was used as an internal standard (IS). O-methylhydroxylamine hydrochloride (purity≥98%) was obtained from J&K Scientific Ltd. (Beijing, China). N,O-bis(trimethylsilyl)trifluoroacetamide with trimethylchlorosilane (99% BSTFA + 1% TMCS) (v/v) was obtained from Sigma Aldrich. Methanol (chromatographic grade) and pyridine were purchased from Shanghai Macklin Biochemical (Shanghai, China). Water was purchased from Hangzhou Wahaha Company (Hangzhou, China).
9
Quantitative Metabolite Analysis Protocol
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MMF with purity of 99%, heptadecanoic acid (purity ≥ 98%), methanol (chromatographic grade), and pyridine were purchased from Macklin Biochemical (Shanghai, China). O-methylhydroxylamine hydrochloride (purity ≥ 98%) was obtained from J&K Scientific Ltd. (Beijing, China). N, O-bis(trimethylsilyl)trifluoroacetamide (containing 1% trimethylchlorosilane) was obtained from Sigma Aldrich (St. Louis, MO, United States). The purified water was purchased from Wahaha (Hangzhou, China). RPMI medium was obtained from (Thermo Fisher Scientific, Waltham, MA, United States).
10
Quantitative Metabolite Profiling Protocol
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High fructose was purchased from Jiangsu Synergy Pharmaceutical & Biological Engineering Co. (Nanjing, China). N,O‐bis(trimethylsilyl)trifluoroacetamide (with 1% trimethylchlorosilane; v/v; Lot No. B‐023) and heptadecanoic acid (as an internal standard, IS, purity ≥98%; Lot No. H3500) were purchased from Sigma‐Aldrich (Saint Louis). O‐methylhydroxylamine hydrochloride (purity: 98.0%; Lot No. 542171) was obtained from J&K Scientific Ltd. (Beijing, China). Pyridine (Lot No. C10486013) was obtained from Macklin Biochemical. (Shanghai, China). Chromatographic‐grade methanol was bought from Thermo Fisher Scientific (Waltham, USA). Pure water was obtained from Wahaha Company (Hangzhou, China).
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