Various tests were used to identify the characteristics of the biosynthesized biofilm-AgNPs. Scanning Electron Microscope (SEM) figure was obtained as described before62 (link) by using JEOL JSM-5300, SEM (Tokyo, Japan) instrument to identify the morphology. On the other hand, Transmission Electron Microscope (TEM) was used according to the previously described protocol63 (link) by applying a single drop of the sample onto carbon copper grids in a JEOL 1230, 1230 JEOL (Tokyo, Japan) to determine the size and morphology. Moreover, EDX was used to detect the percentage of the elements in the sample while relying on JEOL JSM-5300 (Tokyo, Japan) at 20 kV for a 10 mm working distance64 (link). The Zeta sizer and potential of the sample were measured using rapid, non-invasive dynamin light scattering (DSL) in (Zetasizer Nano ZS (Malvern, UK)) according to65 (link) with several dilutions to identify their size. The UV–Vis spectrophotometer (Jenway 7200, Staffordshire, UK) was utilized with a wavelength range of 340–800 nm66 (link). The sample was not sonicated and left to dry, then take the measurement. Distilled water was utilized as a blank. Moreover, the functional groups in the biofilm-AgNPs and biofilm layers were evaluated by Fourier Transform Infrared Spectroscopy (FTIR) analysis using the Agilent system Cary 360 FTIR model, which ranges from 4000 to 400 cm−1.
Jsm 5300
Manufactured by JEOL
Sourced in Japan, United States
The JSM-5300 is a scanning electron microscope (SEM) manufactured by JEOL. It is designed to provide high-quality imaging and analysis of a wide range of materials at the micro- and nano-scale. The JSM-5300 uses a thermionic electron source to generate a focused electron beam, which is scanned across the surface of the sample. The resulting signals are detected and processed to create a high-resolution image of the sample's surface.
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Lab products found in correlation
Jfc 1100e, by JEOL (8 mentions)
Jsm 6360la, by JEOL (3 mentions)
Samdri pvt 3d, by Tousimis (2 mentions)
Jem 1230, by JEOL (2 mentions)
Jem 1400, by JEOL (2 mentions)
Jsm 1400 plus, by JEOL (1 mentions)
Ion sputter jfc 1200, by JEOL (1 mentions)
Jfc 1100e ion sputter, by JEOL (1 mentions)
Dsa100, by Krüss (1 mentions)
Ftir spectrophotometer, by Bruker (1 mentions)
2010 fas tem, by JEOL (1 mentions)
Jem 2100, by JEOL (1 mentions)
D2 208219, by Bruker (1 mentions)
Vitek 2 compact, by bioMérieux (1 mentions)
Desk 5 hp, by Denton (1 mentions)
Zetasizer nano series, by Malvern Panalytical (1 mentions)
Thermo plus tg8120, by Rigaku (1 mentions)
Fine coat ion sputter jfc 1100, by JEOL (1 mentions)
Nexus 670 ft ir spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Sio2 nps, by Malvern Panalytical (1 mentions)
72 protocols using jsm 5300
1
Comprehensive Characterization of Biosynthesized Biofilm-AgNPs
2
SEM Preparation of Cell Samples
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For scanning electron microscopy (SEM), cover plates with adhered cells obtained from the pellet after centrifugation were fixed with a 2.5% glutaraldehyde solution diluted in PBS. They were then rinsed with PBS, postfixed with 1% OsO4 solution for 1 h, rinsed again, dehydrated in graded alcohol solutions (ranging from 30% to 100%) and dried afterward. Gold coating was applied using a sputter coater, and the cells were examined using a JEOL JSM 5300 (JEOL Ltd., Tokyo, Japan).
3
Scanning Electron Microscopy of Herbal Powders
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Scanning electron microscopy (JEOL JSM-5300, Jeol Ltd., Tokyo, Japan) was used for observing the morphology of the powders of SMS products [18 (link)]. The herbs of Sheng-Mai-San herbs were purchased from a traditional herbal store in Taipei. The crushed herbs and cornstarch (Sun Right Co., Ltd., Nantou, Taiwan) powders were filtered through a 60 mesh sieve. For sample preparation, the SMS powder was dried at 40 °C for 24 h. The powders were set on an aluminum holder with glue, and then gold powder was coated on the samples by a gold sputter module for 90 s in a high vacuum evaporator (JFC-1200 Ion Sputterer, Jeol Ltd., Tokyo, Japan). Finally, the coated samples were analyzed under a scanning electron microscope.
4
Ultrastructural Analysis of C. dromedarius IFNε Inclusions
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The recombinant C. dromedarius IFNε inclusion bodies were fixed in a solution of formaldehyde and glutaraldehyde (4:1) and observed and analyzed by transmission electron microscopy (TEM; JEOL-JSM 1400 plus) and scanning electron microscopy (SEM; JEOL-JSM 5300).
5
Morphological Analysis of XSLJZT Herbal Powder
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To observe the morphology of the samples, a scanning electron microscope (SEM) was used. The herbal pharmaceutical products of XSLJZT were purchased from six different pharmaceutical manufacturers in Taiwan. The crushed herbs of XSLJZT were obtained from a Chinese traditional herbal medicine shop in Taipei and formed a raw herbal powder in the National Research Institute of Chinese Medicine, Taipei, Taiwan. These herbs were filtered through a 60-mesh sieve after they were ground by the hammer mill (Hung Chuan RT-04, Taipei, Taiwan). Food-grade cornstarch (Sun Right Co., Ltd., New Taipei, Taiwan) was filtered through a 60-mesh sieve. For sample preparation, the herbal pharmaceutical powder was dried at 45 °C for 24 h in an oven (DO45, DENYNG Instruments Co., Ltd., New Taipei, Taiwan). Then the powder was fixed with double-sided adhesive tape on an aluminum stand, and coated with gold by a gold sputter package for 90 s in a high-vacuum evaporator (Ion Sputter JFC-1200, Jeol Ltd., Tokyo, Japan). A scanning electron microscope (JEOL JSM-5300, Jeol Ltd., Tokyo, Japan) was used to analyze the samples. The raw herbal powder, raw herbal extraction, and cornstarch were investigated in the same manner.
6
Fractured Surfaces Ultrastructure Analysis
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After the cyclic fatigue test, the fractured fragments were subjected to ultrasonic cleaning (Sonic 4G, 40KHz, Sonic, Niš, Serbia) in 70% alcohol to prepare them for ultrastructural evaluation. The samples were mounted to aluminum cylindrical stubs with a fixing agent (Dotite paint xc 12 Carbon JEOL, Tokyo, Japan) and sputter-coated with gold/palladium (JFC 1100E Ion Sputter JEOL). Fractured surfaces were examined by scanning electron microscopy (SEM) (JEOL-JSM-5300, Tokyo, Japan). The ultrastructure of the fracture surface as well as the appearance of initial cracks on the perimeter of the section were analyzed.
7
Scanning Electron Microscopy of Xylanase-Producing Fungi
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The microscopic features of the most promising xylanase-producing fungal strain were investigated under scanning electron microscope (SEM). Dry smears of the fungal strain were coated with approximately 15 nm gold (JEC-1100 E Sputter Coater, JEOL Inc., Pleasanton, CA, USA). After that, the golden coated samples were scanned using SEM (JEOL JSM-5300, JEOL Inc., Pleasanton, CA, USA). The coated slide was accelerated at 30 KV (at room temperature). The digital images of the samples were adjusted and saved for further investigation.
8
Scanning Electron Microscopy of Trichosporon Biofilms
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Scanning electron microscopy was performed with 3 T. asahii and 1 T. asteroides isolates selected based on their biofilm production capabilities. Biofilms were formed on sterile polyvinyl chloride (PVC) strips (surface area, 0.5 cm2), incubated into 24-well polystyrene plates with flat bottoms (Techno Plastic Products, TPP, Switzerland), according to the protocol described previously [36] (link). Briefly, all biofilms formed on PVC strips were fixed overnight at 4°C with a solution composed by 4% formaldehyde and 2% glutaraldehyde buffered with 0.1 M sodium cacodylate at pH 7.2. Subsenquently, biofilms were washed repeatedly with 1% osmium tetroxide buffered with cacodylate for 1 h, then treated with 1% tannic acid for 45 min, washed three times with distilled water for 15 min, and finally, treated with 1% osmium tetroxide buffered with cacodylate for 1 h. Biofilms were dehydrated with a graded series of ethanol washes (critical-point dried in CO2) and coated with gold to be analyzed in the scanning electron microscope JEOL JSM-5300 (Peabody, MA, USA).
9
Preparing Mite Specimens for SEM Analysis
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The preparation of mite specimens for SEM analysis was performed according to the method of Panyarachun et al. [38 (link)]. Briefly, mites were fixed in 2.5% glutaraldehyde in 0.1 M phosphate buffer solution (pH 7.2) at 4°C for 3 h. Specimens were washed three times with the buffer, and dehydrated at 4°C through a graded series of ethanol. Mites were dried using the critical point method, mounted using carbon paste on Al-stub, and coated with gold up to the 400 Ǻ thickness in a sputter-coating unit (JFC-1100 E; JEOL Ltd, Tokyo, Japan). The specimen was examined in a JEOL JSM-5300 (JEOL Ltd, Tokyo, Japan) scanning electron microscope operating at 25 keV.
10
Colloidosomes Surface Morphology Analysis
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Total amount of drug Shape and surface morphology Scanning electron microscopy was done to study the particle surface morphology and shape. The sample for the SEM analysis was prepared by sprinkling the colloidosomes on to one side of double-adhesive stub.
The stub was then coated with gold using Jeol JFC 1100 sputter coater (Jeol Ltd, Tokyo, Japan). The SEM analysis of the colloidosomes was carried out by using Jeol JSM 5300 (Jeol Ltd). The colloidosomes were viewed at an accelerating voltage of 15-20 kV.
The stub was then coated with gold using Jeol JFC 1100 sputter coater (Jeol Ltd, Tokyo, Japan). The SEM analysis of the colloidosomes was carried out by using Jeol JSM 5300 (Jeol Ltd). The colloidosomes were viewed at an accelerating voltage of 15-20 kV.
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