Two syringe pumps (Harvard Apparatus Model ‘11’ Plus 70-2208) were used to inject the silver nanoparticle ink and carrier solvent into a microfluidic Y-junction chip (Cellix Ltd) which has a rectangular channel with width of 200 µm and depth of 50 µm with a hydrophobic wall coating. The fluids exited the chip through a 300 µm inner diameter outlet pin which also acted as a nozzle. The pin was positioned 100 µm above a polyethylene naphthalate (PEN) substrate which was placed on a computer-controlled translational stage (Thorlabs DDSM50). The substrates were treated with ozone-plasma prior to the printing using UV-Ozone cleaner (Ossila). After the printed silver nanoparticle lines were dried on a hotplate at 110 °C for 10 mins.
Uv ozone cleaner
Manufactured by Ossila
Sourced in United Kingdom
The UV-Ozone cleaner is a laboratory equipment designed to clean and activate the surface of various materials. It uses a combination of ultraviolet (UV) light and ozone (O3) to remove organic contaminants from the surface, making it suitable for a range of applications that require a clean, treated surface.
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Lab products found in correlation
6 protocols using uv ozone cleaner
1
Inkjet Printing of Silver Nanoparticles
2
MoO3 Seed Layer Deposition and Annealing
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Different types of substrates such as commercial fluorine-doped tin oxides (FTO) (15 Ω cm−2), p-doped Si/SiO2 wafers (SiO2 thickness: 1 μm) were cleaned using a standard procedure by sonication in acetone (5 min), isopropanol (5 min), and ethanol (15 min), followed by drying in a nitrogen flow. The cleaned substrates were treated with a UV/ozone cleaner (Ossila) for 20 min to form a polar surface to improve the adhesion of the MoO3 seed layer.32 (link) The MoO3 seed layer was deposited on the different substrates using thermal evaporation (Tectra mini-coater) at a base pressure of <2 × 10−6 mbar with applied current and power of 15 A and 1 kW, respectively. The thickness of the film was monitored using a quartz micro-balance with a resolution of ±0.1 nm. Finally, the seed layer coated substrate was annealed at 450 °C for 1 h under ambient atmosphere.
3
Formulation and Printing of PEDOT:PSS Inks
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The printing ink was formulated by blending water-based PEDOT:PSS solution (Clevios™ P VP AI 4083 from Ossila) with semiconductor-grade isopropyl alcohol (IPA) in a vial. The volume ratio of the mixture was set at 3:7 (vol/vol). Pipettes with a volume of 1000 µL were used for this task. To eliminate larger undispersed PEDOT:PSS particles, the solution was passed through a 0.45 µm filter. The resulting polymer ink was then loaded into a syringe, ready for printing onto ITO substrates. The ITO substrates (purchased from MXBAOHENG via Amazon) were precisely cut into rectangular slides of 25 mm × 75 mm. These ITO slides exhibit sheet resistances ranging from 10 to 15 /square, and the water contact angle on the surface of ITO slides measures 77.8°. The slides were ultrasonically cleaned with IPA to ensure surface cleanliness. Subsequently, the substrates underwent a 15-min ozone cleaning process in an Ossila UV Ozone Cleaner to eliminate micro/nano-impurities.
4
Fabrication of Plasmonic TERS Probes
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ATEC-NC and ATEC-CONT Si AFM cantilevers
(NanoAndMore, Germany) were used to prepare contact mode and tapping
mode TERS probes. To increase the refractive index of the surface,25 (link) Si cantilevers were oxidized in a furnace (Carbolite
Gero, U.K.) at 1000 °C for 22.5 h to obtain a SiO2 layer of ∼300 nm. Before metal coating, oxidized cantilevers
were cleaned inside a UV–ozone cleaner (Ossila, United Kingdom)
for 1 h. Probes were then coated with Au (99.99%, Acros Organics)
to a nominal thickness of 100 nm at 0.02 nm/s and a pressure of <10–6 mbar inside a thermal evaporation system (MBraun,
Germany). To prepare contamination-free TERS probes with high plasmonic
sensitivity, the entire thermal evaporation system was placed inside
a nitrogen glovebox (MBraun, Germany) with <0.1 ppm of oxygen and
moisture.
(NanoAndMore, Germany) were used to prepare contact mode and tapping
mode TERS probes. To increase the refractive index of the surface,25 (link) Si cantilevers were oxidized in a furnace (Carbolite
Gero, U.K.) at 1000 °C for 22.5 h to obtain a SiO2 layer of ∼300 nm. Before metal coating, oxidized cantilevers
were cleaned inside a UV–ozone cleaner (Ossila, United Kingdom)
for 1 h. Probes were then coated with Au (99.99%, Acros Organics)
to a nominal thickness of 100 nm at 0.02 nm/s and a pressure of <10–6 mbar inside a thermal evaporation system (MBraun,
Germany). To prepare contamination-free TERS probes with high plasmonic
sensitivity, the entire thermal evaporation system was placed inside
a nitrogen glovebox (MBraun, Germany) with <0.1 ppm of oxygen and
moisture.
5
Quartz Crystal Microbalance Binding Assay
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Before the experiments, Au-coated AT-cut crystals (QSX-301 Biolin Scientific, Q-Sense, Sweden) were immersed in 2% (v/v) Hellmanex solution and rinsed with double distilled water. The gold sensors were further dried using nitrogen flow and cleaned using UV-ozone cleaner (E511, Ossila, Sheffield, UK) for 30 min. The QCM-D experiments were performed using the Q-Sense E4 (Biolin, Q-Sense, Sweden) instrument and AT-cut quartz disks (5 MHz). All the experiments were performed in a buffer solution under a constant flow rate of 50 μL/min at 25°C. Briefly, the gold surface was equilibrated with buffer, followed by neutravidin adsorption (200 μL, 0.2 mg/mL protein solution). 5′-biotinylated, 3′-cholesterol-modified DNA was then used (200 μL, 0.075 pmol/μL) as a binding anchor to liposomes of different lipid compositions as previously described [71 (link)]. All liposome solutions were used at a concentration of 0.2 mg/ml and a final volume of 70 μL. Finally, protein solutions of various concentrations were added at a volume of 500 μL.
6
Fabrication of Zwitterionic Particle Films
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The two particle dispersions were blended to obtain different zwitterionic particle volume fractions (in the dry film): f zwitterionic = 0.05, 0.18 and 0.35, corresponding to f wet zwitterionic = 0.005, 0.016 and 0.033. 200 mL of the corresponding blend were cast on a square glass substrate (18 mm  18 mm) which had been previously been washed with acetone, dried with compressed air and treated in a UV ozone cleaner (Ossila) for 10 minutes. Cast dispersions were dried at different evaporation rates: (i) fast evaporation, under a 250 W infra-red lamp (Intelec) at a distance of 10 cm; (ii) medium evaporation, at 21 AE 1 1C and 50 AE 10% RH; and (iii) slow evaporation, in a high humidity chamber at 21 AE 0.5 1C and Z90% RH. Typical drying times were 7 min (fast evaporation), 3 h (medium evaporation), and 5 days (slow evaporation). The constant high humidity environment was created through heating deionized water at 50 1C on a hot plate in a semi-sealed Perspex box.
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